【作者】 高洁；

【导师】 张喜奎；

【作者基本信息】 福建中医学院 ， 中医临床基础， 2009， 硕士

【摘要】 目的:本课题旨在通过动物实验,研究桃核真武汤与益肾降浊汤对5/6肾切除所致慢性肾功能衰竭大鼠肾小管功能的影响,从细胞分子水平观察两方防治慢性肾功能衰竭的作用机理。方法:采用5/6肾切除所致慢性肾衰大鼠作为研究对象,SD大鼠78只,雌雄各半,随机分为空白对照组8只、假手术组10只和手术组60只。手术组采用两步法制备5/6肾切除CRF模型后,再随机分为病理模型组、依那普利对照组、大黄颗粒对照组、益肾降浊治疗组、桃核真武治疗组,手术四周后,开始六周的治疗。观察治疗后大鼠的一般状况,治疗结束后测定各组大鼠的血清BUN、Scr、β₂-MG及尿β₂-MG、RBP、NAG酶;同时取上述各组大鼠肾脏观察其形态学改变。结果:1.病理模型组大鼠血清BUN、Scr、β₂-MG和尿β₂-MG、RBP、NAG酶水平显著高于正常组（P＜0.01）,说明5/6肾切除大鼠模型存在肾小管功能的损伤。2.桃核真武汤和益肾降浊汤能明显改善5/6肾切除大鼠的一般情况,降低血清BUN、Scr、β₂-MG和尿β₂-MG、RBP、NAG酶水平（P＜0.01）,光镜下显示两方可以改善肾小管-间质病理损伤,效果优于大黄颗粒和依那普利。3.治疗组中桃核真武汤和益肾降浊汤比较,二者无明显差异。结论:桃核真武汤与益肾降浊汤能有效降低5/6肾切除大鼠慢性肾衰模型血清BUN、Scr、β₂-MG和尿β₂-MG、RBP、NAG酶,一定程度上改善肾小管-间质的病理损伤,对大鼠肾切除所致慢性肾衰肾小管-间质损伤的改善有一定的疗效,效果优于依那普利和大黄颗粒;桃核真武汤与益肾降浊汤是防止或延缓肾小管-间质损害至终末期肾衰的有效方剂。更多还原

【Abstract】 Objective:This issue through animal experiments,researchers Taohehenwu and Yishenjiangzhuo Decoction of the 5/6 nephrectomy in Chronic Renal Failure(CRF) caused by renal tubular function in rats,from the cellular and molecular level of both prevention and treatment observed in chronic,renal mechanism of failure.Methods:5/6 nephrectomy rats with CRF as a result of the study,SD rats with 78,were equally divided between male and female,were randomly divided into blank control group,8, 10 sham-operated group and operated group 60.Operation group was prepared using two-step 5/6 nephrectomy model of CRF,and then were randomly divided into model group pathology,enalapril control group,rhubarb granular control group,treatment group Yishenjiangzhuo,Taohezhenwu treatment group,surgery four weeks after the beginning of a six-week treatment.Observed after treatment of the general state of rats,treatment of each group after the determination of serum BUN,Scr,β2-MG and urinaryβ2-MG,RBP,NAG enzyme;At the same time,take the above-mentioned group to observe the morphological changes of kidney.Results:1.Pathologic model of serum BUN,Scr,β2-MG and urinaryβ2-MG,RBP, NAG enzyme level was significantly higher than normal group(P＜0.01),5/6 nephrectomy model of renal tubular function in rats damage.2.Taohezhenwu Decoction and Yishenjiangzhuo Decoction can be significantly improved 5/6 nephrectomized rats in general,lower serum BUN,Scr,β2-MG and urinaryβ2-MG,RBP,NAG enzyme level(P＜0.01),light microscope showed that the two sides can improve the renal tubulepathological interstitial injury,rhubarb particles and better than enalapril.3.Treatment group Taohezhenwu Decoction and Yishenjiangzhuo Decoction comparison,no significant difference between the two.Conclusions:Taohezhenwu Decoction and Yishenjiangzhuo Decoction can be reduced effectively with 5/6 nephrectomy rat model of CRF serum BUN,Scr,β2-MG and urinaryβ2-MG,RBP,NAG enzyme,to some extent to improve the renal tubule-pathological interstitial injury in rats with CRF caused by renal tubular resection-the improvement of tubulointerstitial injury has some effect,more effective than enalapril and rhubarb particles; Taohezhenwu Decoction and Yishenjiangzhuo Decoction is to prevent or delay renal tubular- stromal damage to end-stage renal failure effective prescription.更多还原