【作者】 陈秀丽；

【导师】 刘荣； 王振宇；

【作者基本信息】 东北林业大学 ， 食品科学， 2008， 硕士

【摘要】 红松(Pinus koraiensis Sieb.et Zucc.)是主产于中国小兴安岭的珍贵树种,其种仁油中含有多种极具开发价值的生理活性物质,可食用或用于功能性食品,具有广阔的应用开发前景。本文分别以高脂饲料(20%猪油和80%基础饲料HF)及高脂饲料加松仁油(20%松仁油和80%高脂饲料SHF)饲喂小鼠,记录体重调整饲喂量,于45d和90d测定体脂含量及酶法测定血脂水平,取脑和肝脏制成相应浓度的组织匀浆液测定脂肪酶(LPS)、丙二醛(MDA)、脂褐质(LP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)水平,取睾周脂肪以RT-PCR法测定酰基CoA合成酶(ACS)、脂肪酸结合蛋白(FABP)、脂肪酸转运蛋白(FATP)、激素敏感性甘油三酯脂肪酶(HSL)、过氧化物酶体增殖剂激活受体-γ(PPAR-γ)mRNA水平,主要结果如下:1、试验期内,松仁油组试验动物能量的摄入显著高于正常组和高脂组,虽然试验结束时松仁油组小鼠的体重显著高于正常组(P＜0.05),但与高脂组比体重显著降低(P＜0.05),体脂含量呈降低趋势,说明松仁油具有减体重,降低体脂含量的作用。2、松仁油可以使高脂小鼠血清TG,TC,LDL-C的含量降低(P＜0.01),而HDL-C升高(P＜0.01),提示松仁油具有一定的调节血脂的作用,在调节脂质代谢方面具有积极的意义。3、松仁油组脑和肝脏组织中LPS的活性显著升高(P＜0.01),提示松仁油可通过提高LPS的活性,加速脑和肝脏中脂肪的分解速度。4、松仁油可以同时提高ACSmRNA、FATPmRNA和HSLmRNA的表达,促进脂肪的分解,减少体内脂肪的贮存;同时可以降低PPAR-γ的转录活性,抑制FABPmRNA的表达,进而抑制脂肪的生成。5、喂高脂食物可使小鼠的脑和肝脏CAT、总SOD、GSH-PX活性显著降低(P＜0.01),并随时间延长各个酶的活性呈下降趋势,同时脑和肝脏中MDA和LP的含量显著升高(P＜0.01),提示高脂饲料加速体内脂质过氧化反应进程。松仁油虽不能完全逆转高脂饲料导致的体内抗氧化酶活性降低和脂质过氧化物含量升高,但却可使这种变化得到部分改善,尤其是CAT和总SOD的活性在实验中期和末期显著高于正常组(P＜0.01),提示松仁油可通过提高抗氧化酶系统的活性降低机体内自由基反应,从而提高机体的抗氧化能力。更多还原

【Abstract】 Pinus koraiensis Sieb.et Zucc is a rare tree species of Xiao xing an ling in China. Pine seed oil includes many kinds of developed activity materials, can be edible or used in functionality food, so it has the broad development prospect.This paper respectively feed the mice by high fat feedstuff (80% added 20% lard HF) and added pine-seed oil feedstuff(80% high fat feedstuff added 20% pine-seed oil SHF), record weight to adjust the quantity of feeding, in 45 days and 90 days, measure the body fat content and the enzyme method measurement blood fat level, and also in 45 days and 90 days, takes the brain and liver to make the tissue homogenate and measures MDA、LP、CAT、SOD and GSH-PX level. Takes the fat of periphery testicle, assay ACS、FABP、FATP、HSL、and PPAR-γby RT-PCR. The main results are as follows:1、In the trial period, the energy taking of SHF experiment animal is obviously higher than the normal group and HF(P<0.05). Although when the experiment is over, the body weight of SHF is obviously higher than that of the normal group,but the body weight is more remarkable reducer than that of HF. So the results explained that the pine-seed oil can reduce the body weight and can reduces the body fat content.2、Pine seed oid can make the high fat small mice serum TG、TC, and LDL-C lower(P<0.01), but the HDL-C go up(P<0.01), suggested that pine-seed oil has definite effect on regulating blood fat and has positive significance in fat metabolism.3、The LPS activeness in brain and liver of SHF obviously elevate(P<0.01), so it prompts that the pine-seed oil can accelerate the fat disintegration rate in the brain and the liver by enhancing LPS activeness.4、The pine oil may simultaneously enhance ACSmRNA, FATPmRNA and the HSLmRNA expression, promote fat decomposition, reduce in vivo fat storing; simultaneously may reduce PPAR-γduplication activeness, suppress FABPmRNA the expression, then suppress the fat the production.5、Feeds high fat food to be possible to cause mice brain and liver CAT, total SOD, the GSH-PX activeness obviously reduces(P<0.01), and along with time each enzyme’s activeness assumes the declining trend, simultaneously the brain and the liver’s CAT and the LP content obviously elevate(P<0.01), prompt the high fat feed to accelerate in vivo lipin peroxidization advancement. Although the pine seed oil cannot reverse in vivo oxidation resistance enzyme activity and reducing the content of the lipin peroxide content by the high fat feed, but actually the pine seed oil may partly improve this kind of change, particularly CAT and the total SOD activeness obviously is higher than the normal group in the experiment intermediate stage and the last stage(P<0.01), prompts the pine seed oil to be possible through to enhance the antioxidase system’s activeness to reduce in organism the free radical to respond, thus enhances organism oxidation resistance ability.更多还原