【作者】 苏世平；

【导师】 李毅；

【作者基本信息】 甘肃农业大学 ， 林木遗传育种， 2008， 硕士

【摘要】 本文以红砂和沙拐枣一年生实生苗为研究对象,研究不同培养阶段最佳培养基及激素配比,以及影响快速繁殖的关键性因素,初步建立了一套较为完善的适合红砂和沙拐枣组培快繁的技术路线,为干旱地区植被恢复和种质资源离体保存提供技术支持。研究主要结果为:1外植体选取以一年生5cm以下实生苗为佳,部位以茎尖为佳,茎中部次之,茎基部最差。2外植体消毒用10%的84消毒液浸泡30min后用75%酒精消毒4s、0.15%升汞消毒10min搭配为佳,可以有效降低污染,提高灭菌效率。3在红砂组织培养阶段,最佳初代培养基为MS+6-BA0.05 mg·L^-1+IAA0.01mg·L^-1+AC1.0g·L -1;继代培养基为1/2MS+6-BA0.01mg·L^-1+2,4-D0.05mg·L^-1,蓝光培养;生根培养基为1/2MSG+IAA0.01 mg·L^-1+蔗糖20g·L^-1;愈伤组织诱导培养基为H+6-BA0.05 mg·L^-1;愈伤组织增殖培养基为1/2MS+6-BA0.4mg·L^-1+2,4-D0.5mg·L^-1+NAA0.5mg·L^-1;愈伤组织分化培养基为H+6-BA0.1mg·L^-1+2,4-D0.4mg·L^-1。4在红砂和沙拐枣炼苗移栽过程中,移栽基质以腐质土为最佳,红砂苗成活率可达89.23%,沙拐枣苗成活率可达到90.73%。5在红砂初代培养中褐化程度比较严重,在培养基中添加1.0g·L^-1的活性炭可以有效降低培养物褐化;在继代培养中,不同的光质对丛芽分化有明显的作用,蓝光最好,荧光最差。6在沙拐枣组织培养阶段,最佳初代培养基为MS+IAA0.1mg·L^-1+6-BA0.02mg·L^-1;继代培养基为MS+IAA0.1mg·L^-1+6-BA0.05mg·L^-1;生根培养基为MS+IAA0.1mg·L^-1;愈伤组织诱导培养基为MS+6-BA0.05mg·L^-1;愈伤组织增殖培养基为MS+6-BA0.4mg·L^-1;愈伤组织分化培养基为MS+6-BA0.4 mg·L^-1+2,4-D0.5 mg·L^-1。更多还原

【Abstract】 1-year-old seedlings of Reaumuria Soongorica and Calligonum Mongolicum were used as the research object in this paper,the optimal medium and hormone combination were studied in different culture stage and the key factor effected the rapid propagation of Reaumuria Soongorica and Calligonum Mongolicum, a more perfect technical route was preliminarily established for Reaumuria Soongorica and Calligonum Mongolicum tissue culture, it can provide some technical support for vegetation restoration in drought condition and In vitro conservation of Reaumuria Soongorica and Calligonum Mongolicum.The main experiment results were as the following:1 1-year-old seedlings below 5cm was the best for explants,the part of plant for selecting explants, the stem of plant was the best , the middle of plant was better , the basal of plant was the worst.2 The best explants disinfection process was: 10% 84 disinfection solution immersion explants 30mins,then 75 % alcohol disinfected 4s and 0.15% HgCl2 disinfected 10min,it could be effectively decreased explants contamination and improved the disinfection efficiencily.3 In Reaumuria Soongorica culture stage ,the best initial culture medium was MS+6-BA0.05 mg·L^-1+IAA0.01mg·L^-1+AC1.0g·L^-1,the best Subculture medium was 1/2MS+6-BA0.01 mg·L^-1 +2,4-D0.05mg·L^-1,under blue light,the best rooting culture medium was 1/2MSG+IAA0.01 mg·L^-1,with 20g·L^-1sucrose,the best callus induction medium was H+6-BA0.05 mg·L^-1,the best callus multiplication medium was 1/2MS+6-BA0.4mg·L^-1+2,4-D0.5mg·L^-1 +NAA 0.5mg·L^-1,the best callus differentiation medium was H+6-BA0.1mg·L^-1+2,4-D0.4mg·L^-1。4 Rotten soil was the best for Reaumuria Soongorica and Calligonum Mongolicum transplanting in vitro. the survival rate of Reaumuria Soongorica was more than 89.23%,Calligonum Mongolicum was 90.73%.5 Browning was bad in Reaumuria Soongorica initial culture, addition 1.0g·L^-1AC in medium can effective reduction browning,in subculture, the bud differentiation effect was obviously under different light qualities, blue light was the best , fluorescence was the worst.6 In Calligonum Mongolicum culture stage, the best initial culture medium was MS+IAA0.1 mg·L^-1+6-BA0.02mg·L^-1,the best Subculture medium was MS+IAA0.1 mg·L^-1+6-BA0.05 mg·L^-1, the best rooting culture medium was MS+IAA0.1 mg·L^-1,the best callus induction medium was MS+6-BA0.05mg·L^-1,the best callus multiplication medium was MS+6-BA0.4 mg·L^-1,the best callus differentiation medium was MS+6-BA0.4 mg·L^-1+2,4-D0.5 mg·L^-1。更多还原