【作者】 李长峰；

【导师】 张永清；

【作者基本信息】 山东中医药大学 ， 中药学， 2008， 硕士

【摘要】 目的:对丹参与白花丹参植株的形态特征、光合特性、种子蛋白电泳、叶片过氧化物酶谱、根部性状与重量、化学成分含量及高效液相指纹图谱进行全面系统的研究,为丹参的良种选育及白花丹参的药材质量评价提供基本依据。方法:综合运用药用植物学、药用植物栽培学、植物生理学、生药学等学科的基本手段,观察丹参与白花丹参的植株形态,比较丹参与白花丹参在光合特性、遗传性、药材产量性状、化学成分含量及高效液相指纹图谱等方面的差异。植株形态特征与药材产量性状测定采用测量法,光合速率测定采用光合测定仪,种子蛋白与过氧化物酶谱研究采用凝胶电泳法,浸出物含量测定采用药典法,丹参酮ⅡA和丹酚酸B含量测定采用高效液相色谱法。结果:丹参与白花丹参植株形态非常相近,但株高、花苔长度、果实总数量等指标丹参显著高于白花丹参。丹参植株的光饱和点高较白花丹参高、光补偿点较白花丹参低。丹参与白花丹参种子蛋白中的水溶蛋白、盐溶蛋白、醇溶蛋白及碱溶蛋白谱带基本一致,在谱带条数、谱带位置及清晰程度方面无明显差异。丹参与白花丹参叶片过氧化物酶谱共出现了6条谱带,但只有1条谱带为二者所共有,丹参有3条独立谱带,白花丹参有2条独立谱带。根部长度、直径及折干率丹参与白花丹参相差不大,但根部分支、鲜重、干重均以丹参为高。丹参与白花丹参的水溶性与醇溶性浸出物含量均以丹参较高,而丹参酮ⅡA和丹酚酸B的含量却以白花丹参较高,但4类成分含量之间均无显著性差异。丹参与白花丹参指纹图谱中的主要特征峰、相对保留时间基本一致,峰面积比值大小差异不大。结论:丹参与白花丹参在遗传性上有一定差异,但药材质量基本一致,因此白花丹参药材应归为丹参药材之列,而不宜作为一种新药材对待。丹参净光合速率、根部重量均显著高于白花丹参,生产中还是以种植紫花植株能够获得较高的收益,但白花丹参可以作为种质予以保存。至于白花丹参能否作为丹参的变种或变型对待,有待于深入研究。更多还原

【Abstract】 Objective: The morphological characteristic, photosynthesis character, seed protein electrophoresis, peroxidase zymogram of leaves, root character and root weight, the content of chemical constituent and HPLC fingerprint between Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. were studied systematically, which provide basic foundation for fine germplasm selection of Salvia miltiorrhiza Bge. and the quality evaluation of Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L..Methods: The diversity of morphological character, photosynthesis character, genetic character, the character that showing yield, the content of chemical constituent and HPLC fingerprint between Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. were compared using the basic methods in pharmaceutical botany, medicinal plant cultivation, plant physiology and pharmacognosy. The net photosynthetic rates were measured with CIRAS-1. The plant character and the character that showing yield were measured. The protein band of seed and peroxidase band of leaves were studied by gel electrophoresis. The extractum content was determined in accordance with China pharmacopeia. The content of tanshinoneⅡA and salvianolic acid B were determined by high performance liquid chromatography (HPLC).Results:The morphological characteristic of Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.were similar to Salvia miltiorrhiza Bge., the numerical value of the plant height, flowering woods and the seeds quantity for the latter were higher than that of the former. The light saturation point of Salvia miltiorrhiza Bge. was higher, while its light compensation point was lower. The seed protein electrophoresis band of both Salvia miltiorrhiza Bge.and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. was at equal place, but the peroxidase band of leaves showed marked difference. Both the content of chemical constituent and HPLC fingerprint of Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. were different unobviously.Conclusion : There are certain genetic differences but little quality difference of medicinal material between Salvia miltiorrhiza Bge. and Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.. So, the latter should be calssified as Radiex et Rhizoma Salviae Miltiorrhizae rather than as a new medicinal material. The net photosynthetic rates and root weight of Salvia miltiorrhiza Bge. were significantly higher than that of Salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.. In production, Salvia miltiorrhiza Bge. should be cultivated to achieve more profit, while salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L.could be preserved as a germplasm. Whether salvia miltiorrhiza Bge.var.alba C.Y.Wu et H.W.L. was a variety or a variant to Salvia miltiorrhiza Bge., more research should be done.更多还原