【作者】 陈朝艳；

【导师】 陈效兰；

【作者基本信息】 湘潭大学 ， 分析化学， 2008， 硕士

【摘要】 荷移反应是基于电子给体和电子受体之间的电荷转移而形成荷移络合物的一类反应。物质生成荷移络合物之后,其溶解性、分配系数、结晶形状、溶液颜色、特别是最大吸收波长发生变化,呈现出荷移络合物特有的性质。大多数的药物都含有富电子基团（氨基、苯环、嘧啶基等）)均可作为电子给体。荷移反应用于药物分析而形成的荷移分光光度法具有简便准确,专属性强,较高的灵敏度等优点,不仅适于单一药物含量的测定,更适于复方制剂的测定,因而展示出良好的应用前景。本论文以茜素红和茜素作为电子受体,建立了测定作为电子给体的药物阿昔洛韦和头孢拉定的新方法。本论文主要研究内容如下:1.研究了在水介质中,阿昔洛韦与茜素红在30℃水浴中放置反应75 min后形成1:1型荷移络合物,其λmax=520 nm,表观摩尔吸光系数为1.03×104 L·mol^-1·cm^-1,线性范围为1.0×10-6～2.5×10-4 mol/L,线性回归方程为A=0.02200+0.3873C （10-4mol/L）,相关系数R为0.9995,相对标准偏差为0.80% （n=8）,加标回收率为96.44～100.2%。用于注射用阿昔洛韦含量的测定,结果满意。2.研究了在乙醇-水介质中,阿昔洛韦与茜素在40℃水浴中放置反应60 min后形成1:1型荷移络合物,其λmax=537 nm,表观摩尔吸光系数为7.94×103 L·mol^-1·cm^-1,线性范围为5.0×10-6～2.0×10-4 mol/L,线性回归方程为A=0.08805+ 0.1779C（10-4mol/L）,相关系数R为0.9990,相对标准偏差为0.62% （n=7）,加标回收率为97.78～106.2%。用于片剂中阿昔洛韦含量的测定,结果满意。3.研究了在水介质中,头孢拉定与茜素红在室温条件下即可形成稳定的1:1型荷移络合物,其λmax=520 nm,表观摩尔吸光系数为4.70×103 L·mol^-1·cm^-1。线性范围为5.0×10-6～5.0×10-4 mol/L,线性回归方程为A=0.03570+0.2042C（10-4mol/L）,相关系数R为0.9999,相对标准偏差为0.60% （n=6）,加标回收率为97.00～99.29%。用于注射用头孢拉定含量的测定,结果满意。4.研究了在乙醇-水介质中,头孢拉定与茜素在30℃水浴中反应75 min后形成稳定的1:1型荷移络合物,其λmax=524 nm,表观摩尔吸光系数为2.01×104L·mol^-1·cm^-1。线性范围为2.5×10-6～2.5×10-4 mol/L ,线性回归方程为A=0.1629+0.1920C（10-4 mol/L）,相关系数R为0.9990。相对标准偏差为1.1% （n=7）,加标回收率为91.40～103.6%。用于胶囊中头孢拉定含量的测定,结果满意。更多还原

【Abstract】 Charge transfer reaction belongs to that kind of reaction in which charge transfer complex is formed by charge transfering between electron donor and electron acceptor. After formed charge- transfer complex, substance presents its own character. The dissolution, the distribution, the figure of crystal, the colour of solution has changed a lot. A lot of drugs that are abundant in electrons are used as electron donors. Charge transfer spectrophotometry that is used in pharmaceutical analysis is brief, fast and exact. So it has good far forward.In this paper, UV-Vis spectrophotometry was used to study the charge transfer reaction between medicine and alizarin red or alizar as electron donors. The studies are as follows:One is that aciclovir can react with alizarin red to form 1:1 complex after being placed in the 30℃water bath for 75 minutes, which gives a maximum absorption peak at 520 nm. The molar absorptivity of the complex was 1.03×104L·mol^-1·cm^-1 and the linear range was 1.0×10-6～2.5×10-4 mol/L.The linear equation was A=0.02200+0.3873C （10-4mol/L）, R=0.9995, and RSD was 0.80% （n=8）. If used in the determination of aciclovir for injection, the recoveriey was between 96.44% and 100.2%.The second is that aciclovir can react with alizarin to form 1:1 complex in the presence of alcohol-water solution after being placed in the 40℃water bath for 60 minutes, which gives a maximum absorption peak at 537 nm. The molar absorptivity of the complex was 7.94×103 L·mol^-1·cm^-1 and the linear range was 5.0×10-6～2.0×10-4 mol/L. The linear equation was A=0.08805+0.1779C （10-4mol/L）, R=0.9990, and RSD was 0.62% （n=7）. If used in the determination of aciclovir troche, the recovery was between 97.78% and 106.2%.The third is that cefradine can react with alizarin red to form 1:1 complex in the presence of water at room temperature, which gives a maximum absorption peak at 520 nm. The molar absorptivity of the complex was 4.70×103 L·mol^-1·cm^-1 and the linear range was 5.0×10-6～5.0×10-4 mol/L. The linear equation was A=0.03570+0.2042C （10-4mol/L）, R=0.9999. RSD was 0.60% （n=6）. If used in the determination of cefradine for injection, the recovery was between 97.00% and 99.29%. The forth is that cefradine can react with alizarin to form 1:1 complex in the presence of acohol-water solution after being placed in the 30℃water bath for 75 minutes, which gives a maximum absorption peak at 524nm. The molar absorptivity of the complex was 2.01×104 L·mol^-1·cm^-1 and the linear range was 2.5×10-6～2.5×10-4 mol/L. The linear equation was A=0.1629+0.1920C （10-4 mol/L）, R=0.9990. RSD was 1.1% （n=7）. If used in the determination of cefradine capsule, the recovery was between 91.40% and 103.6%.更多还原