【作者】 聂鸿平；

【导师】 熊炳贤；

【作者基本信息】 南昌大学 ， 外科学， 2008， 硕士

【摘要】 目的:本实验通过研究癌旁正常胃组织、淋巴结转移癌组织及胃癌组织中VCPmRNA(valosin containing protein)的表达规律及其与PCNAmRNA(增殖细胞核抗原)表达的相关性,探讨VCPmRNA在胃癌发生发展过程中的可能作用,为胃癌的诊断、治疗及预后判断提供新的方法和思路。方法:本实验收集南昌大学二附院手术切除并经病理证实的42例胃癌患者标本,运用半定量RT-PCR技术检测胃癌组织、淋巴结转移癌组织、相应癌旁正常胃组织中VCPmRNA、PCNAmRNA的表达情况,分析其与胃癌临床病理因素的相关性,探讨VCPmRNA与胃癌细胞增殖、转移的关系。应用SPSS13.0版统计软件,采用Kruskal-Wallis H、两样本t检验、单因素方差分析、Spearman相关检验、多元线性回归检验进行统计学分析,并以p<0.05作为具有统计学意义的判断标准。结果:1 VCPmRNA、PCNAmRNA在胃癌中的表达及其与临床病理因素的相关性VCPmRNA在胃癌组织、淋巴结转移癌组织、相应癌旁正常组织中积分光比值分别为:1.267±0.368、1.148±0.425、0.380±0.119,PCNAmRNA在胃癌组织、淋巴结转移癌组织、相应癌旁正常组织中积分光比值分别为: 1.415±0.289、1.296±0.360、0.433±0.120,统计学检验发现胃癌组织、淋巴结转移癌组织与正常胃组织中表达分布有显著性差异(Hc =71.31,P值=0.000;Hc =82.41,P值=0.000),胃癌组织、淋巴结转移癌组织较正常胃组织表达显著升高,两者在胃癌组织中的表达丰度与性别、年龄、肿瘤大小及生长部位无关,与肿瘤的分化、浸润深度、淋巴结转移个数及病理TNM分期有相关性。2 VCPmRNA、PCNAmRNA在胃癌中表达的相关性VCPmRNA、PCNAmRNA在胃癌组织中表达存在正相关关系,r=0.631,p值<0.001。结论:1 VCPmRNA、PCNAmRNA表达在胃癌组织及淋巴结转移癌组织中呈高表达,与相对应的癌旁正常胃组织的表达比较明显上调。2 VCPmRNA、PCNAmRNA在胃癌组织中的表达与年龄、性别、肿瘤大小、肿瘤的生长位置无关,而以胃癌的分化、浸润深度、有无淋巴结转移及转移个数相关。3癌组织中VCPmRNA、PCNAmRNA表达呈正相关性,前者高表达可能是上调PCNAmRNA表达的一个因素,促进肿瘤细胞的分裂、增殖,对于肿瘤的发生发展具有重要影响,联合检测两者的表达对预测胃癌患者预后﹑指导治疗有一定临床辅助意义。更多还原

【Abstract】 Objective: To study the expression and distribution of VCPmRNA of gastric cancer、lymphatic invasive cancer and corresponding adjacent normal gastric tissue and its relationship with PCNAmRNA expression of gastric cancer, to investigate VCPmRNA’possible roles in the gastric carcinogenic mechanism and to provide a new method to diagnosis,therapy,and prognosis assessment。Methods: Speciments of gastric cancer、lymphatic invasive cancer and correspond- ding adjacent normal gastric tissue were obtained from 42 cases who had undergone surgical operations of gastric cancer at the second hospitals affiliated to the university of Nan Chang。All samples were confirmed by pathologic diagnosis.Expressions of VCPmRNA、PCNAmRNA were dectected by senmiquatitative reverse transcriptase polymerise chain reaction in 42 specimens of gastric cancer、lymphatic invasive cancer and corresponding adjacent normal gastric tissue,and further we investigated the relationships between the expression of VCPmRNA、PCNAmRNA and the clinicopathologicparmeters.explored the possible role of VCPmRNA in proliferation、metastasis of gastric cancer. The data were analysized by software SPSS13.0 using Kruskal-Wallis H test,Independent-samples T test, One-Way ANOVA test,Spearman’rho test、Linear Regression test,a difference was considered signicance if the p value was below or equal to 0.05。Results:1 Expression of VCPmRNA、PCNAmRNA and the relationships to clinicopathologic- aparameters。The rations of intensities of VCPmRNA/GAPDHmRNA、PCNAmRNA/GAPDHm- RNA of gastric cancer and lymphatic invasive cancer and corresponding adjacent normal gastric tissue were 1.267±0.368、1.148±0.425、0.380±0.119 and1.415±0.289、1.296±0.360、0.433±0.120,respectively.There are significant differences of VCPmRNA/GAPDHmRNA、PCNAmRNA/GAPDHmRNA between gastric cancer、lymphatic invasive cancer and corresponding adjacent normal gastric tissue(Hc =71.31,P=0.000;Hc =82.41,P=0.001),VCPmRNA and PCNAmRNA were shown upregulated in gastric cancer、lymphatic invasive cancer compared to paired normal tissue。Besides,high expression of VCPmRNA and PCNAmRNA were associated with deep tumor invasion、lymph node metastasis、pTNM stage and histological differentiation(p<0.05),but not associated with age、gender、tumor location、tumor size( P>0.05)。2 Correlation among expression of VCPmRNA and PCNAmRNA in gastric carcinomaThere was a significant positive correlation between expression of VCPmRNA and PCNAmRNA in gastric carcinoma(r=0.631,p<0.001)。Conclusions:1 VCPmRNA and PCNAmRNA were over expressed in gastric carcinoma. VCPmRNA and PCNAmRNA were shown upregulated in gastric cancer、lymphatic invasive cancer compared to paired normal tissue。2 VCPmRNA and PCNAmRNA expression were significantly correlated with progression of gastric carcinoma,differentiation grades,depth of tumor invasion and lymph node metastasis. but not associated with age、gender、tumor location、tumor size。3 There was a significant positive correlation between expression of VCPmRNA and PCNAmRNA in gastric carcinoma,high expression of VCPmRNA might be a cause upregulating the PCNAmRNA,and promote tumor celIs to cleavage or proliferate,and play an important role in tumorigenesis and progression。by jointly detecting of the expression of them, might function as helpful markers in predicting the prognosis of and directing the reasonable therapy for gastric cancer。更多还原