【作者】 刘海洲；

【导师】 梅荣军；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2008， 硕士

【摘要】 目的:采用自制的自动推拿器对颈神经根损伤造模后的大鼠进行定量机械振动、按压腧穴和振动经络治疗,通过运用神经电生理和免疫组化方法,观察其对大鼠神经根损伤的修复作用,以探讨其相关的治疗机制,并为临床推拿提供基础理论依据。方法:把144只Wistar大鼠随机分为3组,每组48只,各标记为机械治疗组、NGF治疗组、模型组。每组中又按照处理时间的长短在造模后随机分为4个亚组,每一个亚组分别为12只,各标记为7d、14d、21d、28d亚组。待一周切口愈合后,治疗组采用机械振动按压治疗,对照治疗组采用大鼠臀大肌注射NGF进行治疗;模型组正常饲养不予治疗。在治疗后第7d、14d、21d、28d,分别对大鼠肢体进行直径的测量,以及大鼠患侧臂丛神经离体电生理检测、下颌下腺免疫组化检测、臂丛神经HE染色后光镜观察。结果:14、21、28天机械组肢体直径差率优于模型组（^aP＜0.05、^bP＜0.01）;21天机械组优于NGF组（^cP＜0.01）;28天NGF治疗组优于模型治疗组（^aP＜0.05）。14、21、28天机械组运动神经传导速度优于模型组（^aP＜0.05、^bP＜0.01）;28天机械组优于NGF治疗组,NGF治疗组优于模型组(^acP＜0.05)。14、21、28天起机械治疗组神经干动作电位优于模型组（^aP＜0.05、^bP＜0.01）;21、28天机械治疗组优于NGF治疗组（^cP＜0.05）,NGF组优于模型组（^aP＜0.05）。7天模型组下颌下腺NGF平均灰度指数表达优于机械组和NGF组;21、28天NGF组和机械组优于模型组（^aP＜0.05）。结论:机械振动、按压治疗大鼠颈神经根的损伤与NGF治疗组和模型组相比,能够有效地加快损伤神经的修复,减缓骨骼肌萎缩程度;促进大鼠下颌下腺NGF的分泌。更多还原

【Abstract】 Objective:Using a self constructed mechanical vibration manipulation machine to treat rats with cervical nerve root injury using a treatment method of mechurgical methods.They were then allowed to heal for one week before the various treatment methods were performed.Afterwards,the rats were allocated randomly and equally into three groups that each contained forty eight rats.The first group was treated by manipulation of mechanical vibration and compression,the second group was treated by injecting NGF into the gluteus maximus major of the rats,and the third group which had received no medical treatment was the model comparianical vibration and steady compression of certain acupoints.The results were then analysed using nerve electrophysiology and immunohistochemistry to observe the effect of treatment methods on the recovery of damaged rat cervical nerve root tissue,and to speculate about the mechanism of recovery.Method:Right-sided cervical nerve root injury was induced in 144 Wistar rats by sson group.Each group was then allocated randomly and equally into four subgroups so that each subgroup contained twelve rats.These subgroups were named,7d（7 days）,14d （14days）,21d（21 days）and 28d（28 days）.Each group was sacrificed at the appropriate time,and various parameters were taken.We measured and compared the diameter of both forelimbs,measured the electrophysiology of the cervical nerve root ex vivo,used immunohistochemistry to treat the submandibular gland,and observed the nerve cell ultrastructure under a microscope using HE-stained brachial plexus.Results:In the 14 day,21 day,and 28 day groups,the difference in forelimb diameter of the manipulation of mechanical group was significantly lower than that of the model comparison group（^a P＜0.05,^b P＜0.01）.In the 21 day group,the difference in forelimb diameter in the manipulation of mechanical group was significantly lower than that in the NGF treatment group（^cP＜0.05）.In the 28 day group,the difference in forelimb diameter in the NGF treatment group was significantly lower than that in the model comparison group （^aP＜0.05）.In the 14 day,21 day and 28 day group,the MNCV of the manipulation of mechanical group was significantly faster than that of the model comparison group（^aP＜0.05,^b P＜0.01）. In the 28 day group,the MNCV in the manipulation of mechanical group was significantly faster than that in the NGF treatment group（^c P＜0.05）.In the 28 day group,the MNCV in the NGF treatment group was significantly faster than that of the model comparison group (^acP＜0.05).The MNAP in the 14 day,21 day,and 28 day groups of the manipulation of mechanical group and were significantly higher than that of the model comparison group（^a P＜0.05,^b P＜0.01）.The MNAP of the manipulation of mechanical group was significantly higher than that of the NGF group in the 21 day and 28 day groups（^cP＜0.05）.The MNAP of the manipulation of the NGF group was significantly higher than that of the model comparison group in the 21 day and 28 day groups（^aP＜0.05）In the 7day group,NGF concentration in the rat submandibular gland of the model comparison group was significantly higher than that of the manipulation of mechanical group and NGF group（^a P＜0.05）.NGF concentration in the rat submandibular gland in the 21 day and 28 day groups of the manipulation of mechanical group and NGF group were significantly higher than that of the model comparison group（^a P＜0.05）Conclusion:This study show that mechanical vibration and steady compression of acupoints can effectively prevent muscle atrophy,increase the speed of injured right forelimb function recovery,induce submandibular gland NGF secretion,and induce the recovery of injured cervical nerves.更多还原