【作者】 肖刚；

【导师】 陆杰；

【作者基本信息】 重庆医科大学 ， 生理学， 2008， 硕士

【摘要】 目的:应用电生理学方法研究红霉素对胃肠感觉传入和心脏自主神经系统功能平衡的影响。方法:1.64只SD成年大鼠随机分成8组(n=8):红霉素组:对照组,小剂量(0.1mg/kg)、中剂量(0.5mg/kg)、大剂量(1mg/kg);阿齐霉素组:分组和剂量均与红霉素相同。经颈静脉注射生理盐水、红霉素、阿齐霉素,观察记录注药前和注药后30分钟内膈下迷走神经传入自发放电和扩胃诱发放电两种状态下的变化。2. 32只SD成年大鼠随机分为对照组,小剂量(25mg/kg)、中剂量(50mg/kg)、大剂量(100mg/kg)四组。在注射红霉素前和注射后30分钟内记录心电图,并通过计算机心率变异性(Heart Rate Variability, HRV)软件提取并分析R-R间期,得到HRV参数:平均心动周期(Mhp),平均心率(Mhr),R-R间期标准差(Hsd),相对分散度(Hrd),李雅普诺夫指数(Hle),分维数(Hfd),混顿度(Hcc),超低频带功率0.003-0.04Hz(绝对值APU,相对值RPU),低频带功率0.04-0.14Hz (绝对值APL,相对值RPL),高频带功率0.14-0.5Hz(绝对值APH,相对值RPH),总功率(TPV),APU/APH和APL/APH(RUH,RLH)。结果:1.静脉注射小剂量红霉素和阿齐霉素自发放电和扩胃诱发放电均无显著变化,静脉注射中、大剂量红霉素和阿齐霉素20 min后自发放电和扩胃诱发放电均增强,与相应的对照组和小剂量组间差异显著(P<0.05)。2.静脉注射红霉素25mg/kg组注射前后HRV各参数值无显著变化。静脉注射红霉素50mg/kg、100mg/kg后5-10分钟,Mhp、Hsd、Hrd、Hfd、APU、APL、APH、RPU、RUH、RLH、TPV增加,Mhr、Hcc、RPH降低(P<0.05)。结论:1.红霉素和阿齐霉素能显著增加胃肠感觉传入。2.静脉注射大剂量红霉素心脏自主神经系统稳定性下降,交感与副交感间平衡失调。更多还原

【Abstract】 AIM: To investigate the effect of erythromycin and azithromycin on sensation afferent nerve functions of gastrointestinal tract and the effect of erythromycin on sympathovagal balance.METHODS:1. Sixty-four Sprague-Dawley rats were randomly divided into erythromycin group, azithromycin group, control group, low dose group (0.1 mg/kg), medium dose group (0.5mg/kg) and high dose group (1 mg/kg). Spontaneous afferent nerve discharge and gastric distention-induced afferent nerve discharge of subdiaphragmatic vagus nerve were recorded before and half an hour after intravenous injection of different doses of erythromycin and azithromycinin.2. 32 Adult Sprague-Dawley rats were randomly divided into control group, erythromycin group(low dose group 25 mg/kg, medium dose group 50mg/kg,and high dose group 100 mg/kg). ECG was recorded and RR interval was extracted for HRV analyzing through a personal computer online before and half an hour after intravenous injection of different doses of erythromycin. HRV results were gain after analyzing: Mean of heart period(Mhp), mean heart rate(Mhr), RR interval standard deviation (Hsd), The relative dispersion(Hrd), Lyapunov exeponent(Hle), fractal dimension (Hfd), and Chaosness(Hcc), ultralow frequency band power 0.003-0.04Hz (absolute value APU, relative magnitude RPU), low frequency band power 0.04-0.14Hz(absolute value APL, relative magnitude RPL), high frequency band power 0.14-0.5Hz(absolute value APH, relative magnitude RPH), total power(TPV), APU/APH and APL/APH (RUH, RLH).RESULTS:1. Intravenous 0.1 mg/kg erythromycin and azithromycin had no significant effect on spontaneous afferent nerve discharge and gastric distention-induced afferent nerve discharge of subdiaphragmatic vagus nerve after 20 min. Intravenous 0.5mg/kg and 1 mg/kg erythromycin and azithromycin had significant effect on spontaneous afferent nerve dis-chargeand gastric distention-induced afferent nerve discharge of subdiaphragmatic vagus nerve which was compared with the control and low dose groups (p<0.05).2. Intravenous 25 mg/kg erythromycin had no obvious effect on HRV after 10 min. Intravenous 50mg/kg and 100 mg/kg erythromycin dose had significant effect on HRV after 10 min, (parameters Mhp/Hsd/ Hrd/ Hfd/ APU/APL/APH/RPU/RUH/RLH/TPV increased, Mhr/Hcc/RPH decreased), which was compared with the control and low dose groups (P<0.05).CONCLUSION:1. Erythromycin and azithromycin have significant effects on sensation afferent nerve functions of gastrointestinal tract in rats.2. Erythromycin has significant effects on sympathovagal balance.更多还原