【作者】 赵鹏；

【导师】 贾亚丁；

【作者基本信息】 山西医科大学 ， 眼科学， 2008， 硕士

【摘要】 目的:探讨在激光诱导BN大鼠脉络膜新生血管(choroidal neovascularization,CNV)中色素上皮衍生因子(pigment epithelium-derived factor, PEDF)的表达及意义。方法:雄性BN大鼠36只,右眼为实验眼,左眼为对照眼,行单眼视网膜氩激光光凝,诱导脉络膜新生血管形成。光凝后随机分成三组,每组12只大鼠,分别在光凝后1、2、3周时摘除眼球制作标本,对光凝区进行病理学检查、Ⅷ因子相关抗原(FⅧR:Ag)的免疫组织化学检查,以观测CNV形成。应用免疫组织化学和酶联免疫吸附检测CNV形成过程中PEDF的表达及变化。结果:通过对激光光凝后7天组、14天组、21天组的各组眼球标本做病理学检查和Ⅷ因子相关抗原(FⅧR:Ag)的免疫组织化学检查,光凝后1周开始形成CNV,2到3周时CNV形成逐渐增多。光斑区内FⅧR:Ag阳性染色密度逐渐增加(P<0.05),说明造模成功。通过对各组眼球标本做PEDF的免疫组织化学检查,PEDF阳性染色密度逐渐下降(P<0.05),PEDF与FⅧR:Ag阳性染色密度负相关(r=-0.832,P<0.05)。通过对各组视网膜标本做酶联免疫吸附试验,检测PEDF在各组标本中的含量,各组PEDF的蛋白含量均比对照组少(P<0.05),而且随着时间的延长各组PEDF的蛋白含量呈递减趋势(P<0.05)。结论:CNV形成与PEDF表达量呈负相关,提示PEDF表达不足可能是CNV形成和增生的主要原因之一。更多还原

【Abstract】 Objective : To investigate the expression and significance of pigment epithelium-derived factor in choroidal neovascularization(CNV) induced by argon laser on Brown Norway rat.Methods:There were 36male BN rat, we divide their right eyes into experimental group and left eye for control group. And we performed photocoagulation by argon laser in single eye to induce CNV. Three groups with 12of each were randomly formed. And their eye ball was harvested after 1, 2, and 3 weeks as specimen. We perform pathological examination and immunohistochemistry examination of FⅧR:Ag to detect the formation of CNV. And the expression of PEDF in the formation process of CNV was detected by immunohistochemistry and Enzyme Linked Immunosorbent Assay.Results:CNV formation was started at 1 week after photocoagulation, and gradually increased at 3 weeks. The positive staining density of FⅧR:Ag in light spot region increased gradually (P<0.05), which indicated that the model was successfully estabished. By immunohistochemistry examination of PEDF in each eye ball specimen, the positive staining density of PEDF decreased gradually (P<0.05), PEDF showed a negative correlation with the positive staining density of FⅧR:Ag (r=-0.832,P<0.05). By Enzyme Linked Immunosorbent Assay of each eye ball specimen, the concentration of PEDF in each specimen was detected. All the specimens of protein concentrtion of PEDF were less than the control groups(P<0.05). And as time went by, the protein concentrtion of PEDF in each specimen decreased gradually(P<0.05).Conclusion: The formation of CNV showed a negative correlation with the expression of PEDF, which indicated that the deficiency of PEDF expression maybe a foundamental reason for formation and proliferation of CNV.更多还原