【作者】 辛玲玲；

【导师】 陈曦；

【作者基本信息】 厦门大学 ， 分析化学， 2007， 硕士

【摘要】 生化需氧量（Biochemical Oxygen Demand）简称BOD,是指在有氧条件下,好氧微生物氧化分解单位体积水中有机物所消耗的溶解氧的量,以mg/L表示。在水质环境监测中,BOD是重要的监测项目,是保证水体自净的重要指标之一。目前国内外普遍采用五日生化培养法（BOD₅）,该方法操作较为复杂、对操作人员要求较高、耗时长,不能迅速地反映水体污染状况和反馈处理信息,也不能满足当前环境监测中快速测定的要求,无法及时为环境管理和决策提供科学依据。随着环境污染问题日益严重,建立和发展连续、在线、快速的现场监测体系尤其重要。近几十年来BOD微生物传感器快速测定方法受到广泛关注,并已开始应用于城市污水和天然淡水中BOD的测定,但专门用于海洋污染监测的BOD传感器很少报道。本论文工作利用光纤光化学传感器响应快、检测过程不耗氧和易微型化等优点,利用实验前期工作构建的BOD光化学传感装置,对BOD微生物传感器的核心部件——微生物传感膜的性能进行了实验条件的优化,并在实验室原理样机的基础上进一步研制了海水BOD快速检测仪,BOD快速检测仪不仅体积小,易于携带,而且具有操作简单,高度集成化等特点。本文共分为四章:第一章,文献综述,主要介绍BOD检测的基本原理、发展概况及其相关技术的研究和应用情况。第二章,利用BOD光纤传感装置对微生物传感膜这一BOD微生物传感器的核心部件进行了实验条件的优化,通过对海水耗氧菌种的有效包埋制备对海水中有机物响应时间短、响应线性好,测定准确度高的微生物传感膜。第三章,在实验室原理样机的基础上研制了海水BOD快速检测仪,具体介绍了其原理、构造、使用方法等,并初步对该仪器的性能进行了考察。实验结果表明本仪器响应线性、稳定性良好,通过对实际海水的测定以及同国家标准方法的比对实验证明仪器较适合复杂海水体系的测定,且测定结果与传统的5日法相关性良好第四章,以二甲基二甲氧基硅烷（DiMe-DMOS）和四甲氧基硅烷（TMOS）为共前驱体,对新型氧猝灭荧光探针5,10,15,20—4—（5-氟基）卟啉铂(PtF₂₀Tpp)进行有效包埋,制备了光化学氧传感膜,并对传感膜的光谱性质、响应特性等进行表征。更多还原

【Abstract】 Biochemical oxygen demand (BOD) is the amount of oxygen required for the bio-oxidation of organic pollutants in a solution. BOD is one of the most widely used and important parameter for the estimation of water quality. The authorized method for BOD is the BOD5 method, which requires 5 days of tedious procedures and the experience and skill to obtain reproducible results. As a result, it is not satisfactory with the rapid evaluation for water quality. The development of on-line approaches for BOD determination has been becoming attractive. In the past decade, although fiber optical chemical sensors for the determination of oxygen have been developed owing to their rapid response, without oxygen consuming and difficult to be poisoned, few BOD sensors have been applied to detect the BOD of seawater. In this dissertation, an optical BOD sensor for the application in seawater was introduced. Based on the BOD device set in our laboratory, a portable BOD apparatus was assembled and its characteristics were studied.This dissertation includes four chapters:In chapter I, the principles, developmental routes and the research status of BOD sensors were reviewed.In chapter II, the experimental conditions for the preparation of the BOD sensing film was optimized. A BOD sensing film immobilizing microorganisms from seawater was found to be rapid response, excellent stability and good linearity to BOD in seawater samples.In chapter III, the measurement theory, the structure of a BOD apparatus and its manipulation methods were introduced. The assembled BOD apparatus presented a good linearity, excellent stability and suitable for BOD determination of the seawater.In chapter IV, a new fluorescence indicator, 5,10,15,20-tetrakis (pentafluorophenyl) porphyrin, was synthesized and selected for dissolved oxygen determination. In the oxygen sensing film, dimethyldimethoxysilane.(DiMe-DMOS) and tetramethoxysilane (TMOS) was selected as precursor. Furthermore, the characteristics for oxygen sensing film, including spectrum properties and response performance, were investigated.更多还原