【作者】 何丹；

【导师】 刘榕芳； 肖秀峰；

【作者基本信息】 福建师范大学 ， 无机化学， 2008， 硕士

【摘要】 为了使人工合成的羟基磷灰石（Hydroxyapatite，简称HA或HAp）医用材料更接近于天然生物材料的结构特点且具有更好的性能，近几年，仿生合成的思路在生物医用材料的制备中已有较多体现。在众多HA合成方法中，要使合成得到的HA晶体在功能上完全符合人们的需求，最有发展前景的合成方法是仿生合成。仿生合成的本质是有机基质调制无机矿物的成核和生长，形成具有特殊组装方式和多级结构特点的生物矿化材料。本论文根据生物矿化原理，采用仿生合成法，以多糖为模板，对制备不同形貌HA纳米材料进行研究。本论文以Ca（NO₃）₂·4H₂O和（NH₄）₃PO₄·3H₂O为反应物，在0．5％壳聚糖（Chitosan，简称CS）的双氧水溶液中，成功地合成了宽约为7-8nm，长30-40nm的梭状纳米HA。以Ca（NO₃）₂·4H₂O和（NH₄）₃PO₄·3H₂O为反应物，分别以一定量的硫酸软骨素（Chondroitin Sulfate，简称ChS）和普鲁兰（Pullulan，简称Pu）为模板剂，通过控制模板剂的浓度等条件，采用仿生合成法分别成功地合成了片状和丝状HA。以及在一定浓度的琼脂糖凝胶中，采用双重扩散法，研究了凝胶浓度对HA粒子的大小的影响。利用X-射线衍射（XRD）、傅立叶变换红外光谱（FTIR）、透射电镜（TEM）、扫描电镜（SEM）、热重／差热分析（TG／DTA）、电导率测定（EC）等对所制得的HA粉体进行表征。结果表明多糖分子和HA之间的相互作用（静电匹配、几何匹配）可能是影响HA晶体形貌的主要原因。而琼脂糖凝胶内部的网络结构则是影响HA的晶粒大小和形貌的主要原因。更多还原

【Abstract】 Hydroxyapatite [Ca₁₀（PO₄）₆（OH）₂, HA or HAP] can be widely used as the substitution of bone transplantation, and is a significant bio-medicine material. In recent years, biomimetic synthesis has been widely used in biomedical materials preparation. To get the HA with the function fully in line with people’s needs, the most promising method to synthesize the artificial bone-like synthetical materials is biomimetic. The essence of the biomimetic process lies in mimicking the biomineralization, in which an organic phase provides a template for inorganic crystals to nucleate and grow from supersaturated solution. This paper, based on the principles of biomineralisation, the different shaped HA were synthesised through the biomimetic method with polysaccharides as templates.This study takes Ca（NO₃）-2·4H₂O and （NH₄）₃PO₄·3H₂O as reagents. Spindle shaped hydroxyapatites （HA） 30-40 nm in length and 7-8 nm in width, were synthesised with 0.5%CS as template in 6% （v/v） hydrogen peroxide solution for 7 days by biomineralization. With ChS or Pu as templates, by controlling the concentration of the organics we successfully synthesized the flake 100 nm in length and 30 nm in width and silk shaped HA by biomineralization. The flake-shaped HA was also synthesized in agarose gel, the effect of the gel concentration on the the crystal size was studied.The crystal phase of obtained samples was characterized by X-ray powder diffraction （XRD） analysis, Fourier-transform infrared spectroscopy （FTIR）, Transmission electron microscopy （TEM）, Scanning electron microscopy （SEM）, Thermogravimetric analyzers-microcomputer differential thermal analyzers （TG-DTA） and Electrical conductivity （EC）. It is indicated that the interaction between the HA and polysaccharides may be one of the reasons which influence the nucleation and growth of HA.更多还原