【作者】 王燕；

【导师】 韦保耀；

【作者基本信息】 广西大学 ， 制糖工程， 2008， 硕士

【摘要】 柿为柿科(Ebenaceae)柿属(DiospyrosL)植物,落叶乔木,生于热带和温带。柿含有黄酮、鞣质、胡萝卜素等多种化学活性成分,具有很高的开发价值。本文以广西桂北地区柿子深加工副产品-柿子皮为原料,进行多酚物质提取、纯化和消臭活性等方面研究,得到如下结论:采用传统溶剂浸提法,在单因素的基础上进行正交实验,确定柿子皮多酚物质最佳提取工艺:丙酮体积百分数50%,浸提温度55℃,浸提时间105min,料液比1:16,在此条件下多酚提取率为71.16%。6种吸附树脂静态实验和静态动力学曲线测定结果表明,HP-20大孔吸附树脂对柿子皮多酚物质具有良好的吸附和解吸性能。通过动态试验确定HP-20上样流速为2BV/h,样液浓度为2.91mg/mL,样液pH为4.8。乙醇梯度洗脱研究表明,40%乙醇洗脱所得样品多酚纯度最高为32.04%。微生物实验表明,初步纯化所得三个样品A、B、C有不同程度的抑菌性能,样品B抑菌效果优于样品A和C,并且抑菌活性随样品剂量的增大而增强。样品B对大肠杆菌、金黄色葡萄球菌、酵母菌最低抑制浓度(MIC)分别为:250mg/mL、125mg╱mL、250mg/mL;最低杀菌浓度(MBC)分别为:500mg/mL、250mg/mL、500mg/mL。运用仪器分析和感官测试两种方法研究柿子皮多酚样品除臭效果,结果表明样品对氨气、三甲胺、硫化氢、甲醛、吲哚均有一定的消除作用。样品B对三甲胺的消除效果最好,甲醛最差,这可能样品多酚纯度及臭气本身的结构和理化性质有关。仪器分析法和感官测试法相结合更加准确的说明多酚样品的除臭效果。运用Sephdaex LH-20对样品B进一步纯化,80%乙醇洗脱所得样品B-2活性最高,多酚纯度为53.64%,对三甲胺消除率为62.37%(1mg/mL)。紫外扫描实验结果表明样品有多酚类物质的特征吸收峰;通过高效液相色谱分析,与所选用标品对照,表明样品B-2中可能含有没食子酸、儿茶素、表儿茶素等酚类物质。更多还原

【Abstract】 Persimmion belonging to Ebenaceae DiospyrosL is Deciduous tree.It distributes in torrid and temperate zone.It is abundant in flavone,tannis and Carotene etc which are bioactive components,so it is worthy of developing. This paper mainly conducted the extraction,purification and deodorant effect of polyphenols from persimmon peel.The results were showed that:The experiment used the traditional solvent extraction.Based on single factor tests,thogonal test was done.The optimum extraction conditions were that the concentration of acet was 50%,extraction temperature is 55℃,the extraction time was 105min and the ratio of the persimmon peel to solvent was 1:16.Under the optimal conditions the extraction yield could reach 71.16%.Through the absorption of static and dynamic curve of static studying in the six kinds of macroporous resin.Resoult showed that HP-20 resin had excellent absorption and desorption properties.The dynamic absorption conditions were flow speed 2BV/h,column density 2.91mg/mL and pH4.8. Through the research on gradient elution,it showed that:40%ethanol eluted the purity which could reach 32.04%.Through the bacterial experiments,three samples had effects of restraining bacterium.The sample B had better antibacterial effect than the other two samples.The elimination effect enhanced with the concentration of sample increasing.The minimum inhibition concentration(MIC)of sample B on Escherichia coli,Staphy lococus and Saccharomyces cerevisiae were listed as follows:250mg/mL,125mg/mL,250mg/mL.The minimum sterilization quality fractions(MBC)were:500mg/mL,250mg/mL,500mg/mL.Combining apparatus with sensory analysis Method.The results showed that:the extract of Polyphenol had effect on ammonia,trimethlamin,sulfureted hydrogen,formaldehyde and indole.The sample B had the best effect on trimethlamin and the worst effect on formaldehyde.This might relate Polyphenol purity,the structure and character of the smelt substance.Apparatus and sensory analysis methods could show the effect of deodorization exactly.Through the further purification by Sephdaex LH-20,the Polyphenol purity of sample B-2 was 53.64%.The deodorant rate of it on trimethlamine was 62.37%(2mg/mL).Samples were detected by UV spectrum.After compared to the characterristic absorption of polyphenol,the sample was supposed to contain the polyphenol.By using the High performance liquid chromatography (HPLC),we selected three kinds of phenolics standards to determine small molecular phenolics in camelliia chryscath.The result showed that sample B-2 might containe gallic acid,the caffeine and the table caffeine.更多还原