【作者】 胡慧；

【导师】 薛新波；

【作者基本信息】 华中科技大学 ， 外科学， 2006， 硕士

【摘要】 目的探讨甲状旁腺组织中细胞周期素D1(CyclinD1)及视网膜母细胞瘤易感基因(Rb)的表达及临床意义。方法应用免疫组化技术对36例甲状旁腺腺瘤组织,10例甲状旁腺正常组织及10例甲状旁腺增生组织中cyclin-D1及Rb肿瘤抑制基因的表达进行检测。结果36例甲状旁腺腺瘤中12例有cyclin-D1过度表达(33.3%),30例Rb表达阳性(83.3%);10例甲状旁腺增生中无一例cyclin-D1过度表达,Rb表达10例均为阳性;10例甲状旁腺正常组织无一例cyclin-D1过度表达,Rb表达10例均为阳性,阴性对照无阳性表达。腺瘤组cyclin-D1过度表达明显高于正常组及增生组(P<0.05);正常组与增生组比较差异无显著性(p>0.05)。Rb的阳性表达正常组、增生组和腺瘤组三者之间比较差异均无显著性(p>0.05)。cyclin-D1和Rb在腺瘤中的表达显著相关,cyclin-D1过度表达与肿瘤大小、血清钙水平及甲状旁腺素(PTH)水平无关。结论cyclin-D1的过度表达在甲状旁腺腺瘤发生中起重要作用。Cyclin-D1过度表达导致Rb磷酸化或表达下调而失去细胞抑制效应可能为甲状旁腺腺瘤发生的机制之一。更多还原

【Abstract】 Objective To investigate the overexpression of cyclin-D1 and the expression of Rb supressoroncogene in parathyroid tissues and the clinical significance. Methods The overexpression of cyclin-D1 and the expression of Rb in 36 parathyroid adenoma tissues and 10 proliferative tissues and 10 normal tissues were detected by ABC immunohistochemistry. Results There are 12 overexpression of cyclin-D1(33.3%) and 30 positive Rb expression(83.3%) in 36 parathyroid adenomas; None of 10 proliferative parathyroid tissues is overexpression of cyclin-D1 and all of the 10 proliferative parathyroid tissues are Rb positive expression; none of 10 normal parathyroid tissues is overexpression of cyclin-D1 and all of the 10 normal parathyroid tissues are Rb positive expression. The overexpression of cyclin-D1 was significantly different between normal parathyroid and proliferation and parathyroid adenoma ( p<0.05),and was not significantly different between normal parathyroid and proliferation ( p>0.05). The expression of Rb was significantly related to the overexpression of cyclin-D1 in the adnoma group. The overexpression of cyclin-D1 was not related to the clinical data. Conclusions The overexpression of cyclin-D1 plays an important role in parathyroid adenoma, which leads to Rb decrease or phosphorylate ,Then loss the ability of inhibiting the growth of cells that contributes to the etiology of parathyroid adenoma.更多还原