【作者】 王晓东；

【导师】 黄志华；

【作者基本信息】 华中科技大学 ， 儿科， 2006， 硕士

【摘要】 目的:通过研究异硫氰酸萘酯(α-naphthyl isothiocyanate,ANIT)所致的急性肝内胆汁淤积兔血清、胆汁和肠液中SIgA的变化,进一步探讨肝内胆汁淤积时SIgA对消化道黏膜免疫的影响及意义。方法:本实验选用新生的大耳白兔(日龄30±3天)作为实验对象。39只兔随机分为实验组(n=27)和对照组(n=12),再将实验组平均分成24h、48h和72h三个时相组。实验组动物按200mg/kg一次灌服含4%ANIT的麻油来诱导新生白兔急性肝内胆汁淤积病变。依次采集各时相实验动物的血清、胆汁、肠液和回肠组织标本。采用自动分析仪检测血清中总胆红素(TB),丙氨酸转氨酶(ALT)的值;用免疫放射法检测上述时相血清、胆汁和肠液中的SIgA含量;同时用比色法检测小肠组织中的髓过氧化物酶(myeloperoxidase,MPO)水平。实验结果采用SPSS12.0软件包作显著性检验。结果:①灌服ANIT 24h后血清和胆汁中的ALT和TB升高明显,48h达到高峰,72h时开始下降;②急性肝内胆汁淤积兔血清中SIgA在中毒后24h开始升高(P<0.05),48h和72h较对照组明显升高(P<0.01);而实验组兔胆汁中的SIgA含量在中毒后24h、48h及72h均明显低于对照组(P<0.01);肠液中的SIgA值则没有明显的变化;③急性肝内胆汁淤积兔在中毒后24h、48h及72h内小肠组织中的MPO值与对照组无差异。结论:急性肝内胆汁淤积兔血清和胆汁中SIgA的水平与肝胆系统损伤的程度有关,胆汁淤积程度越重,血清SIgA水平越高,胆汁中的SIgA浓度越低,随着病变的恢复,胆汁中的SIgA浓度增加,而血清中的SIgA水平逐渐下降;急性肝内胆汁淤积的过程中血清SIgA升高的幅度不如胆道梗阻的情况下升高的幅度大。更多还原

【Abstract】 Objective: To study the alternation of SIgA in serum, bile and intestine fluid in experimental acute intrahepatic cholestasis induced by ANIT (α-naphthyl isothiocyanate), and discuss the mechanism and the significance of the phenomena which has taken place in the intrahepatic cholestasis.Methods: 39 newborn rabbits were divided at random into four groups: 3 experimental groups (n=9) and a control group (n=12). A single dose (200mg/kg) of ANIT was administered by gavage to each experimental rabbit to induce intrahepatic cholestasis. The control group was administered with olive-oil. The specimens of serum, bile, intestine fluid and ileum tissue were collected from rabbits at 24h, 48h and 72h after intoxication respectively and detected by SIgA radioimmunologic kit. At the same time, the tissue of ileum was harvested. Spectrophotometric method was used to detect the activity of myeloperoxidase (MPO) of the ileum tissue. After the experiment, we adopted the SPSS software to analyze the significance test of the experimental results.Results:①Compared with the control group, the SIgA in serum of experimental groups were significantly increased (P<0.01) and the concentration of bile SIgA decreased dramatically (P<0.01);②There were no difference of the concentrations of SIgA in the intestine fluid between the control group and each experimental groups (P>0.05). The levels of MPO in the ileum tissues were unchanged too (P>0.05). Conclusions:①Acute intrahepatic cholestasis induced by ANIT resulted in alterations of SIgA in serum and bile, the fluctuation of the SIgA happened during the intrahepatic cholestasis is coincidence with the injury and recovery of the liver. The alternation may be used to estimate the injury of hepatobiliary system②The actual hepatobiliary system damage doesn’t disturb the molecular immune of the small intestine in the short time.更多还原