【作者】 舒小琴；

【导师】 刘曼西；

【作者基本信息】 华中科技大学 ， 生物信息学， 2006， 硕士

【摘要】 蛋白质组学的研究正在成为生命科学领域中继人类基因组测序后的又一个新热点。蛋白质相互作用网络是蛋白质组功能模式研究的重点。经过近十几年的发展,已经产生了大量有价值的科学文献。这些文献在展示实验数据的同时,也包含了研究者的研究思路和研究方法,它们无疑是后续研究者们的重要研究参考。在科研过程中,试验方案的制定和实验方法的选取传统上是研究者通过查阅和比较大量的相关文献,并结合自己的实践经验完成的。但是随着学科发展,海量文献的产生已经使研究者阅读任务日益繁重且难以参考全面。人们迫切需要多种专门化数据库,但是目前尚无此类关于研究路线与研究方法的专门数据库。本文以蛋白质相互作用为对象,收集整理了一定量的权威科学文献,划分出当前蛋白质互作研究的三个主要阶段,并对各阶段所涉及的研究方法,按技术原理进行分类。整个分类体系包括三个大类,即蛋白质的分离纯化技术,目的蛋白质的构建与表达技术和蛋白质相互作用分析技术。每一大类下再设亚类和亚亚类。这种分类系统具有层次性强、易于理解和应用等优点。本文在比较参考了多种生物学数据库的基础上,针对单机操作特点,采取了Linux+apache+MySQL的黄金组合的设计环境,设定好各项参数的配置,参考有关的设计,探索出了一条适合该分类体系的数据库设计途径,成功的设计出了一个初具规模的完整的、使用灵活便捷的蛋白质相互作用研究方法数据库系统。该数据库界面采取利用PHP语言实现的Web界面,具有查询、下载、分类搜索、更新增加数据、文献上传和试验流程图显示等一系列功能。本数据库旨在给研究者们提供一个可以方便地全面了解各种研究思路、试验方案和实验方法的参考平台。它的建成和完善将为相关领域的研究者们设计研究方案提供可靠的参考数据,也为进行集中对比研究提供了方便。更多还原

【Abstract】 The study of the proteome is becoming a new hotspot after the human genome project. Protein-Protein interaction network study is a main subject of the study of the proteome function mode. Through recently tens years’development, a large amount of correlative literatures have been appeared in many famous magazines, while these literatures showing their data results, they also showing their study processes, which may be the important references for the afterward studiers.During the general study work, the making of the experiment project traditionally is through reading and comparing lots of correlative literatures, and then combining the studiers’experiences to complete this job. But now so large amount of the references make this job always so difficult. Then such kinds of experiments methods databases are greatly needed. But,until now, there is no such methods database.In this paper, we collect lots of correlative reference literatures,classify these study methods used in protein-protein interaction into three steps, and sort every step basing their own technology principles. The whole system includes three large kinds: purification of the proteins, analysis of the protein-protein interactions and protein expression technologies. And every technology includes inferior kinds. This classify system has good arrangement and is easy to understand. After referring lots of similar bioinformatics databases system, we use the design combination of Linux+apache+MySQL,and then complete the design of a database of protein-protein interactions study methods.This database will meet the studiers’needs to provide them a flat roof of providing convenient references, it also provides much dependable data information to make the studies to do more profound studies, and greatly convenient them to make other comparing studies.更多还原