【作者】 车玉媛；

【导师】 李培锋；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2007， 硕士

【摘要】 牛磺胆酸(TCA)是牛羊胆汁中的有效成分之一,也是其发挥药理作用的主要成分之一。本文首次研究了TCA对小鼠体内氧自由基代谢的影响。采用黄嘌呤氧化酶法、二硫代二硝基苯甲酸(DTNB)法、紫外分光光度法、硫代巴比妥酸(TBA)法和酶标(ELISA)法分别测定了给药后健康小鼠血浆,肝组织和脑组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)的活力、脂质过氧化产物丙二醛(MDA)的含量及血浆中一氧化氮合酶(NOS)活力的活力;并测定了四氯化碳(CCL4)致急性肝损伤模型小鼠血浆和肝组织中T-SOD以及血浆中NOS活力。结果显示:低剂量TCA能显著提高健康小鼠血浆、肝组织和脑组织中GSH-PX活力以及肝组织和脑组织中CAT活力,显著降低血浆和脑组织中GSH-PX活力;并显著提高CCL4致急性肝损伤小鼠血浆和肝组织中T-SOD活力,显著降低CCL4致急性肝损伤小鼠血浆中NOS活力。高剂量的TCA能显著提高健康小鼠血浆中GSH-PX活力、肝组织和脑组织中T-SOD和GSH-PX活力、肝组织中CAT活力;显著提高CCL4致急性肝损伤小鼠血浆中T-SOD活力,但对CCL4致急性肝损伤小鼠肝组织中T-SOD活力和血浆中NOS活力的影响差异不显著。低、高剂量的TCA能显著降低健康小鼠血浆、肝组织和脑组织中MDA的含量,显著提高健康小鼠血浆中NOS活力。结论:TCA具有显著的抗氧自由基作用,其抗氧自由基作用与其提高体内T-SOD、GSH-PX、CAT、NOS的活力和降低MDA含量有关;TCA对CCL4致急性肝损伤小鼠具有保护作用,其作用与其提高CCL4致急性肝损伤小鼠体内T-SOD和降低NOS的活力有关。更多还原

【Abstract】 Taurocholic acid (TCA) is one of effective component which exists in cattle, goat bile andso on, and it to bring into full play pharmacological action. It is the first time that the effects of TCA on Oxygen derived free radicals were investigated in mice in this paper. The xanthine oxidase method, DTNB method, ultraviolet spectrotometry, barbital acid method and ELISA method were used to determine the activities of total super-superoxide dismutase (T-SOD), glutathioneperoxidase (GSH-Px), catalase (CAT), NOS,and the content of maleicdialdehyde (MDA) in healthy mice, And the xanthine oxidase method and ELISA method were used to determine the activities of total super-superoxide dismutase (T-SOD) ,NOS of plasma and hepatic tissue with acute damage caused by carbon tetracholoride in mice.The results show that TCA can increase the activity of GSH-PX remarkably in plasma, liver and brain at low dose and the activity of CAT in liver and brain, but decrease the activity of GSH-PX in plasma and brain. TCA can increase the activity of T-SOD remarkably in plasma and liver, and degrade the activity of NOS in plasma in acute liver damaged mice by carbon tetracholoride poisonig. The high dose of TCA can increase the activity of GSH-PX in plasma, T-SOD and GSH-PX in liver and brain and CAT in liver remarkably, increase the activity of T-SOD remarkably in plasma in acute liver damaged mice by carbon tetracholoride poisonig. At both low and high dose, TCA can decrease the content of MDA significantly in plasma, liver and brain, but increase the activity of NOS in healthy mice. The results demonstrated that TCA has the noticeable antioxidation, its antioxidation can be due to the increase of activity of T-SOD, GSH-PX, CAT, NOS and decrease of MDA. And TCA can protect mice from acute liver damaged by carbon tetracholoride poisoning, which can be due to the increase of activity of T-SOD in plasma and liver, and the decrease of activity of NOS in plasma.更多还原