【作者】 李莉；

【导师】 李长生；

【作者基本信息】 山东中医药大学 ， 中西医结合临床， 2007， 硕士

【摘要】 目的:通过动物实验观察化瘀克斑方抗动脉粥样硬化形成的作用并探求其作用机理。方法:取雄性健康新西兰大耳白兔40只,随机分为四组:空白组、模型组、对照组、治疗组,每组10只。采用免疫损伤加高脂饲料喂养方法建立新西兰大耳兔动脉粥样硬化模型。在实验不同阶段动脉B超观察兔腹主动脉病变,并测定血脂、血清NO、血浆ET-1水平。光镜下观察各组血管病理变化,免疫组化法测定腹主动脉平滑肌ICAM-1阳性表达。结果:实验8周末,AS兔血清TC、TG、LDL-C、apoB100及ET-1均显著升高,而HDL-C、apoA1/apoB100及NO显著降低(P<0.05,P<0.01)。动脉B超显示AS兔主动脉IMT测值增大,Vp、Vm增快(P<0.05,P<0.01)。实验16周末,治疗组血清TC、TG、LDL-C、apoB100及ET-1水平较模型组显著降低,HDL-C、NO、apoA1/apoB100较模型组显著升高(P<0.05,P<0.01),治疗组腹主动脉B超示:IMT、Vp及Vm均降低(P<0.05,P<0.01)。光镜下观察:治疗组动物主动脉病理改变明显轻于模型组,主动脉ICAM-1表达较模型组显著降低(P<0.05)。结论:化瘀克斑方具有较好的抗动脉粥样硬化作用,这可能与其能有效地降低动脉平滑肌细胞ICAM-1表达、保护血管内皮功能、调节NO/ET-1平衡及血脂水平有关。更多还原

【Abstract】 Objective: To observe the effects of Huayukeban decoction on preventing AS by animal experiment, and investigate the mechanisms of it’s effect.Methods: 40 healthy male New Zealand rabbits were randomly divided into 4 groups: normal group, model group, contrast group and treat group, and each consist of ten. The model of experimental AS rabbits was established by high cholesterol diet and bovine serum albumin injection. At different time, observe the aortic structure by B-ultrasonography, and test the serum lipid、nitric oxide and endothlin-1 level. Observe pathological changes with light microscope, and immunohistochemistry analysis of ICAM-1 positive percentage was performed.Results: At the end of 8 weeks, AS rabbits’TC、TG、LDL-C、apoB100 and ET-1 are higher than before, but level of HDL-C、apoA1/apoB100 and NO are obviously lower(P<0.05,P<0.01). B-ultrasonography of aortic shows: AS rabbits’IMT of aortic are higher, Vp and Vm are faster(P<0.05,P<0.01). At the end of 16 weeks, TC、TG、LDL-C、apoB100 and ET-1 of treat group are obviously lower than model group, level of HDL-C、apoA1/apoB100 and NO are obviously higher(P<0.05,P<0.01). B-ultrasonography of aortic shows: IMT、Vp and Vm of treat group are all lower than model group(P<0.05). Observition with light microscope shows:the aortic atherosclerosis lesion of treat group are obviously decreased, expression of ICAM-1 of aortic are obviously lower than model group(P<0.05).Conclusion: HuayuKeban Decoction has better antiatherogenic effccts and it’s mechanism might associate with lowering expression of ICAM-1 in aortic, preserving endothelial function, modifying the balance of NO and ET-1 and the serum lipid.更多还原