【作者】 姜孝芳；

【导师】 高丽；

【作者基本信息】 新疆医科大学 ， 中西医结合临床， 2007， 硕士

【摘要】 目的:探讨中药新药—辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠血清一氧化氮(NO)及鼻黏膜诱导型一氧化氮合酶(inducible nitric oxidesyntheses,iNOS)mRNA表达的影响,探讨药效机制。方法:用卵清蛋白建立变应性鼻炎大鼠模型,通过对造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用酶法检测血清一氧化氮水平及逆转录-多聚酶链反应(RT-PCR)半定量测定鼻黏膜诱导型一氧化氮合酶mRNA的表达。结果:血清NO:模型组(57.28±3.76)明显高于实验组(29.11±2.63)、阳性对照组(45.80±6.47)和空白对照组(17.40±3.76),(P＜0.05),实验组大鼠的血清NO低于阳性对照组(P＜0.05)。鼻黏膜iNOS mRNA:模型组(1.1899±0.078)明显高于实验组(0.7648±0.061)、阳性对照组(0.9732±0.055),(P＜0.05),空白对照组未见表达,实验组大鼠的鼻黏膜iNOS mRNA的表达明显低于模型组和阳性对照组(P＜0.05)。结论:辛芷鼻敏胶囊可能通过降低鼻黏膜iNOS mRNA的表达及血清NO含量,抑制AR发病过程NO的合成,进而减少嗜酸粒细胞(Eosinophil,EOS)的浸润而控制症状。更多还原

【Abstract】 Objectives: To study the effect of Xinzhi Bimin capsule on the content of nitric oxide in blood serum and expression of iNOS mRNA in nasal mucosa of allergic rhinitis, and identify the mechanism of pharmacodynamic action. Methods: Ovalbumin sensitized rats used as animal of AR were taken orally with the Xinzhi Bimin capsule. Nitric oxide in serum was measured by nitrate reductase. The expression of iNOS mRNA in nasal mucosa of rat were observed by revers transcriptive polymerase chain reaction (RT-PCR). Results: The content of nitric oxide in blood serum: model group (57.28±3.76)was higer than the experimental group (29.11±2.63), positive control group (45.80±6.47)and normal control group (17.40±3.76) (P<0.05) .The expression of iNOS mRNA in nasal mucosa of rat: model group (1.1899±0.078) was higer than the experimental group (0.7648±0.061) positive control group (0.9732±0.055) (P < 0.05), and no expression in normal control group. The content of nitric oxide in blood serum were lower in the experimental group than the model group(p<0.05)and the positive control group, moreover, the expression of iNOS mRNA in nasal mucosa of rat in the experimental group was lower compared to the model group and the positive control group. Conclusions: The result suggests that the capsule of Xinzhi Bimin can reduce the expression of iNOS mRNA in nasal mucosa of rat and down-regulating the content of nitric oxide in blood serum and treat the symptom of AR in rat.更多还原