【作者】 李太元；

【导师】 金香子；

【作者基本信息】 延边大学 ， 病理学与病理生理学， 2007， 硕士

【摘要】 目的：实验研究大蒜素对棘阿米巴超微结构的影响。方法：用无营养琼脂培养基从土壤中分离棘阿米巴，用PYG培养液培养。将棘阿米巴用10ug／ml、100ug／ml、1000ug／ml、10000ug／ml组的大蒜素处理不同浓度的大蒜素处理，对照组加等量的PYG，在28℃培养24h，在透射电镜下观察其超微结构损伤情况。结果：浓度为10ug／ml组滋养体转化为包囊，未观察到超微结构的损伤；100ug／ml组对棘阿米巴的超微结构产生轻度破坏，胞膜完整，部分线粒体出现变性、肿胀；1000ug／ml以上组棘阿米巴的结构产生严重破坏，可观察到胞膜发生皱缩破坏，胞质疏松，细胞器严重损伤，线粒体肿胀，空化，嵴断裂或消失，内外膜结构不清，部分核膜双层结构不清晰，胞质内空泡消失，部分可见调亡小体。结论：大蒜素浓度100ug／ml以上时可损伤棘阿米巴超微结构，对棘阿米巴有杀伤作用。更多还原

【Abstract】 Objective:To detect the effect of Allitridium against to Acanthamoeba spp. In vitro.Methods:A strain of Acanthamoeba was isolated from soil and cultured in PYG. The Acanthamoeba was cultured with 10ug/ml, 100ug/ml, 1000ug/ml, 10000ug/ml difference concentrations of Allitridium influence of Allitridium on the morphological feature, and ultrastructures was tested.Results: At concentration of 10ul/ml Allitridium forced the Acanthamoeba trophozoites change to. At concentrations of 100ul/ml Allitridium, we were detected light lesion that degeneration and tumefaction of mitochondria. The Allitridium concentration was more than 1000ul/ml, that Allitridium effectively destroyed the ultrastructures of the Acanthamoeba.Conclusions:At concentration of 1000ul/ml Allitridium was effectively destroying Acanthamoeba trophozoites and cystes.更多还原