【作者】 白晓琴；

【导师】 杨建一；

【作者基本信息】 山西医科大学 ， 遗传学， 2007， 硕士

【摘要】 目的:分别从形态学、细胞学及分子水平检测三氯化铝对雄性小鼠生殖系统的毒性作用,以及硒对铝暴露致雄性生殖毒性的拮抗作用,为防治铝暴露所致雄性（男性）生殖损害提供科学实验依据。方法:本实验采用染色体畸变（chromosome aberration,CA）、单细胞凝胶电泳技术（single cell gel electrophoresis,SCGE）,精子畸形（sperm aberration）、精子核荧光染色（fluorescent staining sperm nucleus）和睾丸组织病理切片（pathological section of testis）,从不同层面探讨铝对雄性小鼠生殖毒性的影响及硒对铝生殖毒性的拮抗作用。结果:（1）铝的生殖毒性:铝暴露可使小鼠体重增长值减小（P＜0.05）;小鼠初级精母细胞染色体畸变率升高（P＜0.05）,且有量效关系;彗星实验CASP图像分析结果显示,各剂量组各分析指标与阴性对照组均有统计学意义（P＜0.05）;小鼠精子畸形率升高（P＜0.05）;小鼠精子核未成熟率增高（P＜0.05）,且有明显的剂量-效应关系;睾丸病理切片可见随铝剂量的增加,睾丸间质扩大,曲细精管管腔变窄,各级生精细胞及支持细胞数量减少,管腔内精子较少或缺如。（2）硒对铝致生殖毒性的拮抗作用:中剂量硒可以部分拮抗铝所致的小鼠体重增长值减小（P＜0.05）;低中高剂量硒可以部分拮抗小鼠精子畸形率升高（P＜0.05）,小鼠精子核未成熟率增高（P＜0.05）,以及彗星实验除Olive尾矩（OTM）外的各分析指标的变化（P＜0.05）;低中剂量的硒可以部分拮抗彗星实验OTM分析指标的变化（P＜0.05）;睾丸病理切片可见各干预组各级生精细胞及间质细胞数量较铝组有所增加,管腔内可见许多精子。结论:（1）三氯化铝暴露可致雄性小鼠睾丸细胞DNA损伤和初级精母细胞染色体畸变率、精子畸形率及精子未成熟率增高以及睾丸组织结构损伤。（2）亚硒酸钠可以部分拮抗三氯化铝暴露所致的雄性小鼠睾丸细胞DNA损伤和初级精母细胞染色体畸变率、精子畸形率及精子未成熟率增高以及睾丸组织结构的损伤。（3）亚硒酸钠的拮抗作用和硒剂量及拮抗对象不同有关,0.2、0.4mgNa₂SeO₄/kg/d对睾丸细胞有同等作用的拮抗效果,而0.4mg Na₂SeO₄/kg/d对精子损伤的拮抗效果优于0.2、0.8mg Na₂SeO₄/kg/d的拮抗效果。更多还原

【Abstract】 Objective:The objective of this research was to detect aluminium-induced reproductive toxicity and antagonism of selenium on aluminium-induced reproductive toxicity by morphology,cytology and molecular level.This study was also to provide the evidences for the prevention arid treatment of reproductive toxicity induced by aluminium exposure.Methods:From different aspects,this essay approaches the reproductive toxicity of aluminium and the antagonistic effect of selenium on that in male mice by using chromosome aberration,single cell gel electrophoresis,sperm aberration,fluoresccnt staining sperm nucleus and pathological section of testis.Results:（1）Male reproductive toxicity induced by aluminum:the decreased variation of body weight as well as testicular weight were recorded in mice treated with aluminum alone, which was accompanied by an increase of rate of chromosome aberration,rate of sperm aberration and rate of immaturity sperm;CASP image analyze of comet assay shows that every analytic index of all dosage groups shows statistical significance compared with negative groups;pathological section of testis shows that aluminum can damage the structure of testis.（2）The antagonistic effect of selenium on aluminium-induced Reproductive Toxicity: Se given resulted in partial recovery of the reproductive parameters above studied （P＜0.05）;furthmore the antagonistic effect of selenium is relevant to the dosage.the middle-dose Se is the optimization.Conclusion:（1）Aluminium trichloride exposure could damage testicle cell DNA and increase rate of chromosome aberration,rate of sperm aberration,rate of immaturity sperm and damage the structure of testis.（2）Selenium could antagonize partly aluminium -induced male reproductive toxicity.（3）The antagonistic effect of selenium is relevant to the dosage and object,0.2 and 0.4mg Na₂SeO₄/kg/d groups show the equivalent effect of antagonism on testicle cell, 0.4mg Na₂SeO₄/kg/d group possesses the optimal effect of antagonism on sperm damage.更多还原