【作者】 祝平；

【导师】 杨维仁；

【作者基本信息】 山东农业大学 ， 动物营养与饲料科学， 2007， 硕士

【摘要】 本研究以植物小肽为研究对象,将哺乳阶段分别饲喂对照日粮及含有2%血浆蛋白、2%植物小肽和4%植物小肽日粮的4个处理PIC五元杂交仔猪,于21±2日龄统一断奶。在25±2日龄时从四个处理分别选出健康无病、体质量相近的75头仔猪,随机分为3个重复,统一饲喂保育日粮进行试验研究。通过饲养试验、比较屠宰试验以及结合血液生化指标、肠道组织结构、消化酶活性测定等,系统研究了植物小肽对断奶仔猪生产性能影响及其机理。主要研究结果如下:研究哺乳期使用植物小肽对断奶仔猪生产性能的影响,结果表明:与对照组相比,血浆蛋白组(P >0.05)、2%植物小肽组(P >0.05)和4%植物小肽组(P <0.05)均提高了仔猪日增重;试验全程的料重比,血浆蛋白组较高(P >0.05),2%植物小肽组(P >0.05)和4%植物小肽组较低(P >0.05);血浆蛋白组(P >0.05)、2%植物小肽组(P >0.05)和4%植物小肽组(P <0.05)均降低了仔猪腹泻指数。与血浆蛋白组相比,哺乳期补饲日粮中使用4%植物小肽提高了断奶仔猪日增重(P >0.05),降低了腹泻指数(P <0.05)。研究哺乳期使用植物小肽对断奶仔猪血液生化指标和免疫性能的影响,结果表明:与对照组相比,使用植物小肽对提高血清中的lgA与lgM含量有促进作用,4%植物小肽组显著提高了lgG含量(P < 0.05)。与血浆蛋白组相比,哺乳期补饲日粮中使用4%植物小肽提高了断奶仔猪的血糖浓度(P <0.05)和血清总蛋白浓度(P >0.05),降低了血浆尿素氮浓度(P <0.05)。研究哺乳期使用植物小肽对断奶仔猪肠道组织结构及消化酶系统发育的影响,结果表明:与对照组相比,使用4%植物小肽提高了十二指肠、空肠和回肠的绒毛高度(P >0.05)和绒腺比(P <0.05),减小了隐窝深度(P <0.05);提高了断奶仔猪胰脏胃蛋白酶活性(P < 0.05),十二指肠脂肪酶和淀粉酶活性(P < 0.05),空肠中淀粉酶和胃蛋白酶活性(P >0.05)。研究哺乳期使用植物小肽对断奶仔猪肠道微生物及消化道pH的影响,结果表明:与对照组相比,哺乳期日粮中使用植物小肽,对减少断奶仔猪肠道内大肠杆菌和沙门氏菌的繁殖,增加断奶仔猪肠道内有益菌属乳酸菌的繁殖有一定作用。植物小肽的添加有提高胃、十二指肠、回肠、盲肠pH的趋势。本试验条件下,哺乳期补饲日粮中使用植物小肽能够刺激断奶仔猪肠道发育,使断奶仔猪肠道绒毛高度增加,隐窝深度变浅,能够提高断奶仔猪的生长,促进机体营养代谢,这种趋势随植物小肽使用量的增加而提高。更多还原

【Abstract】 This research centered on the effects of plant mini-peptides on performance and mechanism of weaned piglets(PIC five-cross breed weaned piglets). It was systematically studied by the feeding test and comparative slaughtering test, and the determination of serum biochemical indices, intestinal morphology and digestive enzyme activity. In the study, the control group diets, and 2%spray-dried plasma protein (SDPP), 2%plant mini-peptides and 4% plant mini-peptides in the sucking period diets respectively were added. The test piglers at the 21±2th day of age were weaned and its foundation, with the 25±2th day of age, to choose 75 heads in each groups, were allotted into 3 replicates, the experimental to used the same feeds.The results showed:Research of plant mini-peptides added in the sucking period on performance of weaned piglets. The results showed that, the ADG was increased in the groupⅡ(P >0.05), groupⅢ(P >0.05), groupⅣ(P <0.05). The F:G on whole range was increased in groupⅡ(P >0.05), and was reduced in groupⅢ(P >0.05)and groupⅣ(P >0.05). The diarrhea rate was reduced in groupⅡ(P >0.05), groupⅢ(P >0.05), groupⅣ(P <0.05) compared with control group. After employment 4.0% plant mini-peptides in diet , the ADG was increased (P >0.05)and reduced diarrhea rate(P <0.05) compared with the groupⅡ.Research of plant mini-peptides added in the sucking period on serum biochemical indices and immune performances of weaned piglets. The results showed that plant mini-peptides had no significant improvement on the concentration of IgA and IgM, and 4%plant mini-peptides added in diets had significant improvement on concentration of serum IgG(P <0.05)compared with control group. After employment 4.0% plant mini-peptides in diet, the BS(P <0.05), the STP was increased(P >0.05)and the SUN was decreased(P <0.05)compared with the groupⅡ. Research of plant mini-peptides added in the sucking period on intestinal morphology and digestive enzyme activity of weaned piglets. The results showed that, after employment 4.0% plant mini-peptides in diet, the VH(P >0.05)and the VH/CD was increased(P <0.05), the CD was reduced on duodenum, jejunum and ileum in 4% plant mini-peptides groups(P <0.05). And the activity of PPS in pancreas (P < 0.05), LPS and AMS in duodenum(P <0.05), PPS and AMS in jejunum was elevated(P >0.05)compared with control group.Research of plant mini-peptides added in the sucking period on intestinal flora and enteron pH of weaned piglets. The results showed that the plant mini-peptides added in diets could reduced the multiplication of Escherichia coli (E.coli) and salmonella, and increase the amount of lactobacillus in intestinal compared with control group. After plant mini-peptides was added in diets, have a trend of improving enteron pH of stomach, duodenum, ileum and caecum.After employment plant mini-peptides could improve the development of small intestinal. The villus heights of intestinal were longer and the crypt depths were lower of weaned piglets, the growth performance and nutrition metabolism was improved. This tendency became more evident when the adding rate of plant mini-peptides was elevated.更多还原