【作者】 樊莲莲；

【导师】 刘金荣；

【作者基本信息】 石河子大学 ， 药理学， 2007， 硕士

【摘要】 目的:本课题主要目的是筛选白刺果实总黄酮的最佳提取工艺;分析测定不同成熟期白刺果实及叶中黄酮的含量;对总黄酮进一步的提取分离,并对分离的黄酮单体化合物进行结构鉴定和结构测定;研究和探讨白刺果实有效提取部位的调血脂、抗氧化作用及白刺总黄酮和白刺果油的抗氧化作用。方法:采用紫外分光光度法测定总黄酮含量,单因素实验确定白刺总黄酮的最佳提取条件,并用正交实验确定各因素的影响大小;采用最佳提取工艺对不同时期的白刺果实和叶所含黄酮量进行比较;聚酰胺柱层析和葡聚糖凝胶柱层析对白刺总黄酮进行分离得馏分,用聚酰胺膜鉴定;分离的单体经红外光谱、氢谱、碳谱、质谱测定并进行解析;复制高脂大鼠模型,评价白刺果实醇提取物的调脂和抗氧化作用,复制亚急性衰老小鼠模型,评价白刺果油和总黄酮的抗氧化能力。结果:乙醇浓度、温度、次数、料液比(w/v)、时间对白刺果中总黄酮的提取均有影响;成熟果实所含的黄酮量相对较高;白刺醇提取物、总黄酮和果油对血清和肝脏的各项指标均有影响,肝脏的脂肪变性也有所缓解;经聚酰胺柱层析和葡聚糖凝胶柱层析分离出两种黄酮类单体化合物。结论:1白刺果中总黄酮的最佳提取工艺为乙醇75%,料液比为1:7,温度85℃提取四次,每次2h。2不同成熟期的白刺果实黄酮的含量以成熟果实为高。3所分离的黄酮单体Quercetin和Kaempferol据文献报道也有较强的抗氧化性。4白刺果实醇提取物具有调血脂和抗氧化的作用。5白刺总黄酮和果油均具有较强的抗氧化性。更多还原

【Abstract】 Object: This topic is purpose that optimal extraction condition of total flavonoids in fruit of Nitraria is determined. Screening content of flavonoids in different mature time of fruit of Nitraria and its leaf. Abstraction total flavonoid of Nitraria, identification monomers of abstraction.the ethanol Extraction of fruit of Nitraria is evaluated on regulating lipid and increasing anti-oxidative ability. Meanwhile, total flavonoids and fruit oil is evaluated on increasing anti-oxidative ability.Methods: determination of total flavonoids in fruit of Nitraria by UV spectrophotometer, Single factor experiment was conducted to optimize the extraction process of total flavonoids in fruit of Nitraria .The influence of each factor was measured in orthogonal experiment. Compare with content of total flavonoids between in different time fruits of Nitraria and its leaf by optimize the extraction process. Abstraction total flavonoid of Nitraria by Polyamide and sephadex column chromatography,]identification by Polyamide film; monomers of abstraction were determined by infrared ray、nuclear magnetic resonance and mass spectrometry to analyze. Reproduction model of high lipid, in order to evaluate ability of regulating lipid and increasing anti-oxidative in the ethanol Extraction of fruit of Nitraria . Reproduction model of subacute aging mice, in order to evacuate ability of increasing anti-oxidative in total flavonoids and fruit oil of Nitraria.Results: The concentration of ethanol、temperature、number of times、w/v and times are effecting extraction of flavonids ; content of total flavonoids of mature fruit is relatively high ; the ethanol Extraction of fruit of Nitraria、total flavonoid and the fruit oils were effected different guideline ,The degeneration of hepatic fatty can be modified. Two monomers were obtained through olyamide and sephadex column chromatography.Conclusion:1 The highest extraction rate is 75% ethanol、85℃、1:7(w/v) and extracting 4 times (2h for each time).2 content of total flavonoids mature fruit of Nitraria is relatively high.3 monomers of Nitraria-Kaempferol and Quercetin increase anti-oxidative ability from references.4 the ethanol Extraction of fruit of Nitraria has a satisfactory effect on regulating lipid and increasing anti-oxidative ability.5 total flavonoids and the fruit oils increase anti-oxidative ability.更多还原