【作者】 黄蕾；

【导师】 谷文萍；

【作者基本信息】 中南大学 ， 神经病学， 2007， 硕士

【摘要】 目的：动脉粥样硬化性脑梗死(atherosclerotic cerebral infarction，ACI)的发病具有明显的遗传倾向，寻找ACI的候选基因具有重要意义。本试验旨在探讨湖南地区汉族人群过氧化物酶体增殖体激活受体r2(PPAR-r2)基因Pro12Ala多态性，及其与ACI及血脂水平的关系。方法：本研究筛选190例ACI患者及200例非脑血管疾病人群(均为湖南地区汉族人群)为研究对象。用聚合酶链反应—限制性片段长度多态性方法分析PPARr2Pro12Ala多态性，并测定总胆固醇(TC)、空腹血糖、甘油三脂(TG)、血压、体重指数(BMI)、颈动脉内中膜厚度(IMT)等。结果：ACI组和正常对照组PPARγ2基因P／P型频率分别为0.900租0.884，P／A型频率分别为0.095和0.116，PPARγ2基因频率分布在两组间无显著性差异(P＞0.05)。ACI组中，PA／AA型较P／P型BMI有降低趋势，但未达统计学水平(P=0.61)；血压、血糖以及血清总胆固醇、TC、TG在PP和PA／AA型之间，无显著性差异(P＞0.05)；P／A+A／A型颈动脉IMT较P／P型显著增高(P＜0.05)。结论：1、PPARr2基因Pro12Ala多态性可能不是湖南地区汉族人群动脉粥样硬化性脑梗死发病的遗传因素；与ACI患者的血脂水平亦无明显关系。2、PPARr2基因Pro12Ala多态性可能与动脉粥样硬化硬化的发生和颈动脉内中膜增厚有关。更多还原

【Abstract】 OBJECTIVE: To investigate the relationship between proliferatoractivated receptorγ2 (PPARr2) gene Pro12Ala polymorphism andatherosclerotic cerebral infarction in Hunan population.METHODS: Pro12Ala of PPARr2 in 190 patients withatherosclerotic cerebral infarction and 200 non- cerebral infarctioncontrol subjects were analyzed by PCR-RFLP and electrophoresis, Andtheir data of TG, TC, HDL, LDL, fasting glucose, blood pressure, BMIand IMT datas were collected and reviewd.RESULTS: Allele frequencies of Pro12Ala mutation of PPARγ2Pro12Ala polymophism in the atherosclerotic cerebral infarction groupwere PP 0.900 and PA 0.095; and PP 0.884 and PA 0.116 in the normalcontral group. No significiant difference was found in distribution ofgenotype between these two groups (P＞0.05). Distriubition of frequenciesof each genotype of Pro12Ala variant of PPAR-γ2 was not significantlyassociated with BMI, fasting glucose, lipid, blood pressure, and lipidprofiles. In the atherosclerotic cerebral infarction group, subjects withPPARγ2 Allele had higher carotid IMT (P＜0.05).CONCLUSION: The Pro12Ala mutation in PPARγ2 is notassociated with atherosclerotic cerebral infarction, but the variant ofPPAR-γ2 might promote the increase of carotid IMT in patients suffering from atherosclerotic cerebral infarction.更多还原