【作者】 陈丽君；

【导师】 杨润德；

【作者基本信息】 河北农业大学 ， 预防兽医学， 2007， 硕士

【摘要】 鸽Ⅰ型副粘病毒病，又称鸽瘟(也有人称之为鸽新城疫)，是鸽的一种急性传染病，来势凶猛，流行期长，发病率和死亡率高；以下痢和神经症状为主要特征，给养鸽业造成了巨大的经济损失。近年来我国各地相继使用鸡新城疫疫苗来控制本病的流行，但成效不够显著，仍可造成30％的饲养鸽发病死亡。而且，目前国内外也尚无有效的药物预防和治疗方案。本研究对试验室保存的一株鸽Ⅰ型副粘病毒分离毒(HT-40株)经鸡胚接种多代培养后使其成为鸡胚适应株，采用蚀斑纯化技术在鸡成纤维细胞上纯化出大(HT40-1)，中(HT40-2)，小(HT40-3)三种大小不同的蚀斑，其中大斑直径2～2.2mm，中斑1.5～1.8mm，小斑0.8～1.2 mm，通过对以上三个纯化株理化特性检测，MDT，ICPI，IVPI及免疫原性等一系列生物学特性实验，证明，小蚀斑纯化株属于中偏弱毒性。经免疫原性试验，安全试验、效力试验、证明HT40-3弱毒株安全性高、免疫原性好、效力强、对雏鸡无不良反应，试验鸽接种后，能够有效抵抗强毒攻击，可作为候选疫苗株，为研制预防鸽Ⅰ型副黏病毒病专用疫苗提供籽种。本研究利用细胞病变抑制法测定鸽Ⅰ型副粘病毒(HT-40株)对黄芪多糖、干扰素、聚积胞、利巴韦林、软骨素、胶原蛋白六种药物的体外敏感性；用中性红染料吸收法测定鸡胚成纤维细胞(CEF)活性的方法对病毒侵入细胞及在细胞中复制时六种药物的敏感性进行评定；用病毒滴定法(Reed-Muench法)对药物与病毒的直接作用进行评定。筛选出黄芪多糖、聚肌胞、干扰素具有较强的抑制鸽Ⅰ型副黏病毒的侵入作用；黄芪多糖、聚肌胞具有较强的抗病毒在CEF中的复制作用；而且，在一定范围内药物效果与其浓度呈正相关，表明药物必须有合适的剂量才能发挥最佳疗效。本研究通过鸽体内试验，筛选出聚肌胞、利巴韦林、黄芪多糖均有一定的预防和治疗作用，预防保护率分别为100％，35％，15％，治愈率分别为25％，15％，40％，表明预防效果优于治疗效果，聚肌胞预防效果最好，黄芪多糖治疗效果最好。编制出药物预防鸽Ⅰ型副黏病毒病的程序应用于临床。更多还原

【Abstract】 Pigeon Paramyxovirus 1 (PPMV-1), so-called pigeon plague, caused by ND virus (NDV), is a serious illness of poultry. It has a high morbidity and mortality. The clinical signs were characterized by bilateral conjunctivitisand neurological. It’s the biggest trouble maker of the development of thePoultry industry, in recentyears, ND bacterion was used to cmtrol the prevalence,but,the effect was not prominence,30%feeding Pigeon was dead.Again, it has not effective medication and prevention in abord.Chickembryo inoculability and culture were used to make the PPMV-Ⅰ(HT-40) to adapting virus in chickembryo.Three different size plaques(HT40-1, HT40-2, HT40-3)were depurated from PPMV-Ⅰ(HT40)by using plaque depuration technique. The diarneter of the three plaques were 2～2.2mm, 1.5～1.8mm and 0.8～1.2mm, MDT, ICPI, IVPI .Immunogenicity and other biological proper test proved that the small plaque’ s toxicity was weaked and have good immunogenicity, it can be developed to vaccinal strain.It was proved that the HT40-3 has high security, immunity, efficacity by immunoprophylaxis test,security test and efficacity test,and it has not reaction to chicking .After inoculating.pigeon can defect virusattact,so that ,HT40-3 can be developed to special PPMV-I bacterion.Otherthen, the CPE inhibition test was used to detect the sensitivity effect of PPMV-Ⅰto the six medicine of ragscanth, poly: c, inter feron, ribavirin, chondroitin and collagen. The CEF cellular activity was tested by Neutral Red Assay to evaluate the viral sensitivity effect of the PPMV-Ⅰto medicine when virus invading CEF and reproducing in the CEF.The Reed-Muench test was used to evaluate the effect of the six medicine on direct resisting virus.The result showed that tragscanth,poly:c and interferon inhibited the virus invadeing cell significantly, ragscanth, poly:c,interferon ,ribavirin and chondroitin inhibited the virus reproducting in the cell significantly, and all the six medicine can inhibited the virus directly.In addition there were certain dose-effect relationships.Poly:c, ribavirin and ragscanth have defent and treatment to PPMV-Ⅰby using sensitive effect test,defectiveprotectraterate was 100%,35%, 15% ,curerate was 25%, 15%,40%.It was proved that defectiverate excelled curerate, interferon have the best cureeffect.Medcine defective process was weaved and used to clinic.更多还原