【作者】 司英奎；

【导师】 马晓燕；

【作者基本信息】 辽宁中医药大学 ， 中医内科， 2007， 硕士

【摘要】 目的：通过对脂肪肝大鼠肝脏脂多糖受体CD₁₄mRNA、脂多糖结合蛋白LBPmRNA表达及肝脏病理检测，观察中药肝脂消煎剂对脂肪肝大鼠的治疗效应，并探讨其作用机制。方法：采用高脂饮食和白酒灌胃，经8周建立脂肪肝大鼠模型，造模成功后用肝脂消煎剂治疗，并设东宝肝泰对照组。治疗6周后取肝组织。观测：（1）肝脏Kupffer细胞CD₁₄mRNA；（2）肝组织LBPmRNA；（3）肝组织病理。结果：①高脂饮食加白酒灌胃8周可建立以慢性脂肪肝、脂肪性肝炎为主要病理表现的大鼠脂肪肝模型。②肝脂消煎剂能降低大鼠肝脏Kuppfer细胞CD₁₄mRNA表达水平，其作用明显优于东宝肝泰组（P＜0.01）。③肝脂消煎剂能降低大鼠肝脏LBPmRNA表达水平，其作用明显优于东宝肝泰（P＜0.01）。④肝脂消组肝脏炎症计分显著降低，同时肝组织病理结果显示，肝脂消煎剂具有良好抗脂肪肝，治疗脂肪性肝炎作用，明显优于东宝肝泰（P＜0.05）。结论：肝脂消煎剂治疗脂肪肝的效应及药效学机制为：1、减轻肝细胞炎症反应；2、抑制内毒素血症引起的肝脏Kupffer细胞CD_14mRNA及肝脏LBPmRNA表达。更多还原

【Abstract】 Objective:To observe the therapeutic effect of Ganzhixiao apozema and approach its mechanism of action by detecting the expression of liver’s CD₁₄ mRNA and LBP mRNA, and hepatic tissue’s pathological section in FLD Rats.Methods:High fat diet and distillate spirit were lavaged to the rats for 8 weeks to make the fatty liver rat model. Then rats were divided into four groups: normal control group, model control group, Ganzhixiao apozema treated group and Dongbaogantai treated group. After 6 weeks’treating, we obserbved the following markers: （1）CD₁₄mRNA of Kuppfer’s cell; （2）LBPmRNA of hepatic tissue; （3） pathological section of hepatic tissueResults:①Fatty liver rat model was erected by feeding high fat diet and lavaging distillate spirit for 8 weeks, whose patho-appearance mainly was chronic fatty liver and fatty hepatitis.②Expression of CD₁₄mRNA of Kuppfer’s cell was obviously depressed by Ganzhixiao apozema, and this effect was superior to Dongbaogantai’s（P＜0.01）.③Expression of LBPmRNA of liver was obviously depressed by Ganzhixiao apozema, and this effect was superior to Dongbaogantai’s（P＜0.01）.④Infl activity Accumulated points of liver degraded obviously in Ganzhixiao apozema treated group,and observation results of pathology of liver showed that Ganzhixiao apozema can take effect of anti-fatty liver, and treating fatty hepatitis（AH）, and the effect was superior tO Dongbaogantai’s （P＜0.05）.Conclusion:The pharmacodynamics and therapeutic function of Ganzhixiao apozema decocting prescription to fatty liver as the following:①Lightening the inflammatory reaction of hepatic cell;②Inhibiting the expression of CD₁₄ mRNA of Kuppfer’s cell and LBP mRNA of liver that caused by endotoxemia.更多还原