【作者】 高晋生；

【导师】 宋爱英；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合临床， 2007， 硕士

【摘要】 肝癌居我国癌症中的第二位，肝癌预后差，复发率、转移率均很高。肝癌的血管生成对于肝癌的发生、侵袭、转移具有重要的作用。VEGF是恶性肿瘤细胞产生的最主要的促血管生成因子。因此，VEGF可以评价抗血管治疗药物的生物活性及疗效。目的：建立小鼠腹水型肝癌H₂₂模型，通过给予模型鼠平调饮灌胃评价其作用。方法：用H₂₂腹水肝癌株于右腋皮下种植致移植性肝癌建H₂₂肝癌腹水瘤荷瘤小鼠模型。将模型鼠随机分成4组，通过给模型鼠分别灌胃生理盐水、平调饮、平调饮+5-Fu和5-Fu。观察肝癌瘤体大小、胸腺指数、脾脏指数、抑瘤率、生存期和VEGF肝癌及癌周组织中的表达，并作统计学分析。结果：平调饮组肝癌平均瘤体、VEGF值减小，胸腺指数、脾脏指数、抑瘤率（48.6％）及生存期（168.3％）增高。结论：平调饮通过促进肝癌细胞分化成熟、抑制肝癌细胞增殖、提高免疫力、降低肝癌组织和癌周组织中VEGF表达，而对小鼠腹水型肝癌H₂₂肿瘤发展起抑制作用。更多还原

【Abstract】 Hepatocellular carcinoma （HCC） as the second most common cause of death from cancer in China. Poor prognosis of HCC is mainly due to its high recurrence and metastasis.Angiogenesis,a neovascularization process during which endothelial cells of the preexisting capillaries proliferate and migrate to form new vascular tips,is critical for the growth, invasion and metastasis of HCC.VEGF is the major angiogenic growth factor. Accordingly, it can be assumed that the effects of antiangiogenic agents on VEGF would reflect their antiangiogenic bioactivity and efficacy.AIMS:To establish mouse hepatoma H₂₂ and to study the effects of Pingtiaoyin on the tumor.METHODS:The models of mouse hepatoma H₂₂ were made by vaccination of transplanted tumor in the right axilla.The mouse hepatoma H₂₂ were divided into 4 groups:0.9% NaCl,Pingtiaoyin,Pingtiaoyin+5-Fu,5-Fu respectively.The diameter of transplanted tumor, inhibition rates, thymus and spleen index ,the rates of life prolongation and the expression of VEGF in carcinoma and peri-carcinoma tissues were observed. Statistical analysia was made.RESULTS:The diameter of the tumor and the expressions of VEGF in carcinoma was reduced, thymus and spleen index ,the inhibition rates and the life prolongation rates were upgraded after treatment of Pingtiaoyin .CONLUSIONS:Pingtiaoyin can inhibit the growth of tumor in mouse hepatoma H₂₂ through increasing the reversion of hepatocarcinoma cells,reducing the proliferation of hepatocarcinoma cells, improving the immune function and downregulating the expression of VEGF in carcinoma and pre-carcinoma tissues.更多还原