【作者】 杨晓磊；

【导师】 曹林奎； 陆贻通；

【作者基本信息】 上海交通大学 ， 农药学， 2007， 硕士

【摘要】 复合污染研究已经成为环境科学发展的重要方向之一,多环芳烃(polycyclic aromatic hydrocarbon , PAHs)与重金属是土壤环境中重要的污染物,开展两者复合污染的研究对生态环境保护和治理有重要的意义。本文以PAHs:荧蒽、菲和苯并[a]芘以及重金属:Cd和Zn为目标污染物,采用生物富集法,从重金属-多环芳烃复合污染土壤中筛选PAHs降解菌,并对其降解特性进行研究,以期为复合污染土壤中多环芳烃生物修复提供科学依据。主要研究结果如下:以PAHs为唯一碳源并在重金属胁迫情况下,通过富集培养,从多环芳烃-重金属土壤中,分离纯化得到一株荧蒽优势降解菌。经16S rDNA和系统进化分析,鉴定为克雷伯氏菌属(Klebsiella),命名为JU1,该菌株最适培养条件为30℃,pH为7.0,接种量为10%。对获得的降解菌生长特性进行研究发现,在重金属的胁迫下,菌株仍然能较好的生长,外加碳源葡萄糖对菌株生长有促进作用。用高效液相(HPLC)仪测定降解菌的降解率,结果表明,JU1菌株对荧蒽有较高的降解率,在培养6 d后,达到最高降解率90%以上,JU1菌株对荧蒽、菲和苯并[a]芘降解性能比较:荧蒽>苯并[a]芘>菲。PAHs的浓度对降解率有一定影响,经测定,当浓度为更多还原

【Abstract】 The interacted pollution has been one of the important aspects of the environment science. The polycyclic aromatic hydrocarbon (PAHs) and heavy metals are the main contamination in soil. Now it is significant for people to study how to prevent the interacted pollution of the PAHs and heavy metals in the soil environment.In this study we chose Polycyclic Aromatic Hydrocarbon (PAHs), including Fluoranthene、Phenanthene and Benzo[a]pyrene, and the heavy metals, including Cadmium、Zinc, as target pollutants. The bacteria string was acquired form the soil that polluted by PAHs and heavy metals, and it could degrade PAHs efficiently. Then a further research was carried out on the string to study degradation characteristics. This paper aims to open doors to new ideas and directions for biodegradation of the PAHs in interacted pollution’s soil. Results are as follow:更多还原