【作者】 薛辉；

【导师】 杨世杰；

【作者基本信息】 吉林大学 ， 药理学， 2006， 硕士

【摘要】 杞菊地黄方是已有200年临床应用历史的(源自清·董西园撰《医级》一书)传统配方,由清代医家在六味地黄(熟地、山茱萸、山药、泽泻、牡丹皮、茯苓)的基础上配伍菊花、枸杞子组成。杞菊地黄软胶囊是在杞菊地黄丸的基础上结合现代科学技术开发的新型制剂。六味地黄方增强免疫功能的药理作用已经被现代药理学研究所证实。本研究的目的就是确证杞菊地黄软胶囊制剂在免疫调节方面的作用,为此本文进行了如下研究:1、采用环磷酰胺法制备小鼠免疫抑制模型,测定各剂量组小鼠的T、B淋巴细胞转化功能,以YAC-1为靶细胞测定NK细胞杀伤功能,并用脾细胞上清刺激C57BL/2小鼠胸腺细胞增殖法测定免疫抑制小鼠脾细胞IL-2的活性,以脾细胞上清吞噬靶细胞L929的方法测定TNF的生物学活性。2、环磷酰胺法制备小鼠免疫抑制模型,以营养肉汤培养基诱导腹腔巨噬细胞的生成,并以吞噬中性红的方法测定小鼠腹腔巨噬细胞的吞噬活性。更多还原

【Abstract】 With the development of modern medicine, it has been recognized that the disorder of immune system will bring lots of diseases, as well as it have intimate relations with ageing and the occurrence of tumor, hypertension and diabetes mellitus in aged people.According to the theory of traditional Chinese medicine, the occurrence, development and turnover are determined by the rise and fall of the healthy qi and pathogen. It has intimate relations with the immune function of organism. So that“supporting healthy energy to eliminate evils”were raised as the major treatment principle. Traditional Chinese drugs are mainly natural drug, their drug properties are mild, and they have few adverse effects. Traditional Chinese drugs have distinct pharmacodynamic action on tuning the balance of the whole organism and enhancing the ability of opposing diseases of the organism. It has been proved that ginseng, astragal, ganoderma, medlar and other 200 sorts of traditional Chinese drugs that can support healthy energy or eliminating evils have favorable immunoloregulation action. They can regulate every component element of the immune function, for instance,to improve the function of immune organ, to promote the development and differentiation of immunocell. They can promot nonspecific immunity, e.g. enhance barrier defense function, elevate the phagocytosis of mononuclear macrophage and更多还原