【作者】 车建；

【导师】 唐琳；

【作者基本信息】 四川大学 ， 植物学， 2006， 硕士

【摘要】 藏红花(Crocus sativus L．)系鸢尾科番红花属多年生草本植物，又称番红花(本草纲目)、西红花，主产于欧州及中亚地区，我国有少量栽培，以进口为主。藏红花具有很强的活血化瘀和散郁开结的功效。近年的研究发现，藏红花具有良好的抗癌活性，而且几乎无毒副作用。其疗效可靠，货源稀少，价格昂贵，某些不法商贩制假售假，严重影响了药材质量及用药的安全性。市场上常见伪品及掺伪品有：菊科植物菊花(Chrysanthemum．chanetii．)；莲须，系睡莲科植物莲(Nelumbo nucifera Gaertn．)的干燥雄蕊经染色而成；禾本科植物玉米(Zea mays L．)的须染色假冒藏红花；红花(Garthamus tinctorius L．)染色制作的伪品等。 为了更快速、准确的鉴别出藏红花的真伪，我们进行了以下研究： 1．比较研究藏红花与其易混物种ITS序列的差异和规律，寻找藏红花的分子鉴定方法。 从正品藏红花以及番紫花两个园艺品种中提取总DNA，用ITS序列通用引物扩增，扩增产物克隆测序；莲须、菊花、玉米、红花的ITS序列从GenBank中获得。首次测得并报道藏红花以及番紫花两个园艺品种的ITS全长序列，总长度分别为658bp、634bp和633bp，包括ITS1、5.8S和ITS2区，GenBank注更多还原

【Abstract】 Crocus sativus L. is a valuable plant of traditional Chinese medicine for many human diseases. Its stigmas contain not only expensive spice and pigment, but also bioactive components that can against cancer with little side-effects from the latest research. The very low yield of Crocus sativus L. and strict requirement for habitat result in its shortage for medicinal material and high price in the market. Some iniquity businessmen produce and sell the impurities of Crocus sativus L. which affect the quality of Crocus sativus L. and the safety of using. The impurities possibly contain something fake made of Zea mays L. and Carthamus tinctorius L., the dry androecium of Nelumbo nucifera Gaertn., Chrysanthemum chanetii. et al.We’ve done those studies to distinguish the true saffron from the fake fast and exactly:1. To find the patterns of the rDNA ITS sequence variation of Crocus sativus L. 、 Chrysanthemum chanetii. 、Nelumbo nucifera Gaertn. 、Zea mays L. and Garthamus tinctorius L. and to establish the molecular biological method for the identification of Crocus sativus L. and the others, we do these follow jobs.更多还原