生物系统中玻璃化转变的初步研究

【作者】 李亚维；

【导师】 孙东平；

【作者基本信息】 南京理工大学 ， 有机化学， 2006， 硕士

【摘要】 玻璃化保存技术是生物活性物质保存的一种新的有效技术，其应用日渐广泛。因此本文首先对甘油、1，2-丙二醇碳酸酯的玻璃化转变情况进行了测量，验证了音频内耗仪研究液体力学谱的方法，然后对纤维素的系列浓度溶液和几种玻璃化保存所用的低温保护剂进行初步的研究。此外还对海藻糖用于纤维素酶的玻璃化保存进行了简单的研究。 本实验的研究结果如下： 1．对甘油的实验结果表明，音频内耗仪测量结果与DSC方法结果基本吻合。此方法可以有效的应用于液体力学谱的测量，同样也能为液体玻璃化转变的测量增加一种新的方法。对1，2-丙二醇碳酸酯的实验进一步证明了此方法的有效性。 2．多种低温保护剂的实验表明，本实验方法可以有效地用于低温保护剂的玻璃化转变研究中，但低温保护剂保存效果与测量结果之间的具体联系仍需要进一步的研究。 3．纤维素酶的玻璃化保存实验表明，在冻干、通风干燥、液体保存的情况下，海藻糖对酶活性都能起到很好的保护作用。在通风干燥过程中，20％的海藻糖可以有效地保存酶的活性。更多还原

【Abstract】 Vitrification is a new effective way to preserve bioactive materials and is applied abroad. So the work in this thesis firstly focuses on the vitrification of glycerol and propylene carbonate using mechanical relaxation instrument. Then vitrification behavior on the cellulose solution with different concentration in DMAC/LiCl and some cryoprotectants (CPAs) were preliminarily studied through the instrument. Finally this thesis has some attempt that trehalose was used to the cellulase conservation. The main results were as follows:1. Through glycerol and propylene carbonate experiment, the results showed that mechanical relaxation instrument was an effective way in liquid samples study on vitrification transit.2. Vitrification behaviors of some CPAs were determined using the mechanical relaxation instrument. The results of tests revealed this way can be used in vitrification study of CPAs, but the relationship between conservation effects and determination results has yet been on further studies.3. Using freeze-drying, ventilation dryness or keeping liquid state, trehalose could keep activities of enzyme. Adding trehalose with concentration of 20% could have a good effect on keeping enzyme activities, but that with a higher concentration could lead to an opposition effect. This was probably due to the trehalose crystallization.更多还原