【作者】 张晓彦；

【导师】 任素莲；

【作者基本信息】 中国海洋大学 ， 海洋生物学， 2006， 硕士

【摘要】 利用组织学与组织化学染色方法，对栉孔扇贝外套膜的形态结构，粘液细胞的类型分布及DNA、RNA含量，磷酸酶的活性等进行了研究。研究结果表明，栉孔扇贝外套膜具有双壳贝类外套膜的典型特征，即由中央膜与边缘膜组成，边缘膜具有三个突起，分别为生壳突起、感觉突起及缘膜突起。组织结构包括内外上皮层、结缔组织与肌纤维。不同部位的外套膜，其上皮组织的形态结构、结缔组织与肌纤维含量等有差异，这与其结构和功能密切相关。另外，上皮层中分布有几种不同类型的粘液细胞。 AB-PAS(pH2.5)染色反应显示，栉孔扇贝外套膜粘液细胞主要分为四种类型：Ⅰ型：AB-PAS染色呈红色，AB阴性，PAS阳性，含有中性粘液物质；Ⅱ型：AB-PAS染色呈蓝色，AB阳性，PAS阴性，含有酸性粘液物质；Ⅲ型：AB与PAS都呈阳性，PAS阳性反应较强，含有以中性粘液物质为主的混合粘液物质；Ⅳ型：AB与PAS都呈阳性，AB阳性反应较强，含有以酸性粘液物质为主的混合粘液物质。粘液细胞分布于上皮层内，结缔组织内未见分布。 粘液细胞内含物含有丰富的RNA，并具有较强的酸性磷酸酶(ACP)和碱性磷酸酶(AKP)酶活性。更多还原

【Abstract】 The microscopic anatomy of the mantle of Chlamys farrei is described through histological and histochemical techniques. The typical structure of the mantle of bivalve consisting of a middle zone and a marginal zone is observed. There are three protuberances in the marginal zone. The mantle is composed of inner epithelium, outer epithelium, connective tissue and muscular fiber. Several kinds of mucous cells are distributed in the epithelium and connective tissue.The types and distribution of mucous cells in the epithelium are observed and analyzed by the methods of AB-PAS (pH2.5) . Based on the observation the mucous cells are divided into four types: type I, pure red, PAS positive and AB negative, the mucous cells include neutral mucopolysaccharide; type II, pure blue, PAS negative and AB positive, the mucous cells include acid mucopolysaccharide; type III, PAS positive more than AB, the mucous cells include mixed mucopolysaccharide; type IV, AB positive more than PAS, the mucous cells include mixed mucopolysaccharide. The results show that the mucous cells exist in the epithelium, but the types and density of mucous cells vary in different parts.Not only plenty of RNA but also the strong activity of ACP and AKP are observed in the mucous cells.更多还原