【作者】 陶淑娟；

【导师】 毕开顺；

【作者基本信息】 沈阳药科大学 ， 药物分析学， 2004， 硕士

【摘要】 蒙药那如-3是由草乌（制）、荜茇、诃子三味药材组成的丸剂，是蒙医常用成药。本文对其质量评价方法进行研究。 采用薄层色谱法(采用硅胶GF₂₅₄板，在紫外灯254nm下观察)对那如-3丸中胡椒碱、没食子酸进行了定性鉴别。以苯-乙酸乙酯-丙酮（v∶v∶v=7∶2∶1）为展开剂，胡椒碱的R_f值为0.40；以氯仿-乙酸乙酯-甲酸（v∶v∶v=7∶2∶1）为展开剂，没食子酸的R_f值为0.30。 分别采用冷浸法、回流提取法和超声提取法对那如-3丸中的乌头碱、中乌头碱和次乌头碱的提取效果进行了考察，结果显示超声提取法最好。研究了超声提取法中溶剂的影响。确定最佳提取工艺为：那如-3丸粉末用10％氨水润湿后，用乙醚作为溶剂超声提取。 采用反相高效液相色谱法（Hypersil ODS色谱柱），对那如-3丸中三种药材的主要成分：草乌（制）中的3种乌头类生物碱（乌头碱、中乌头碱和次乌头碱）、荜茇中的胡椒碱及诃子中的没食子酸5种成分进行了定量分析：以甲醇-水-氯仿-三乙胺（v∶v∶v∶v=60∶40∶2∶0.1）为流动相，在254nm进行检测，次乌头碱、乌头碱和中乌头碱在0.3～1.5mg·mL^-1范围内峰面积与浓度呈线性关系，其回归方程分别为A₁=2.084×10⁶c+3.120×10⁴、A₂=1.827×10⁶c+1.680×10⁴、A₃=1.618×10⁶c-3.970×10⁴，相关系数r分别为0.9996、0.9998和0.9998，平均回收率分别为101.3％、101.0％和100.9％，RSD分别为1.4％、1.9％和1.2％；以甲醇-水（v∶v=77∶23）为流动相，在更多还原

【Abstract】 The extraction process and quality assessment of the Mongolian recipe Naru-3 pill, which is composed of Radix Aconiti Kusnezoffii Preparata, Fructus Piperis Longi and Fructus Chebulae were studied in this thesis.Piperine and Gallic acid in Naru-3 pill were identified by TLC with the clear component dots and very good reproducity. Piperine was identified using silica gel GF254 with benzene-ethyl acetate-acetone （v:v:v=7:2:1） as developer and observed at 254nm using ultraviolet lamp. The R/ of Piperine was 0.40. Gallic acid was identified using silica gel GF254 with chloroform-ethyl acetate-formic acid （v:v:v=7:2:l） as developer and observed at 254nm using ultraviolet lamp. The R/ of Gallic acid was 0.30.A reliable extraction method for the target components from the drug Naru-3 was set up and purification of the extract for the quantitative determination of the aconitine alkaloids: aconitine, hypaconitine, mesaconitine, was analyzed by RP-HPLC with Hypersil C₁₈ column at room temperature. The mobile phase consists of methol-water-chloroforrn-thriethylamine （v:v:v:v=60:40:2:0.1） with a flow rate of 0.8mL·min^-1 and the injection volume of 4μL. The external standard was used and the wavelength of the detector was set at 254 run. The linear range was 0.3～1.5mg·mL^-1, and correlation coefficients were 0.9996, 0.9998, 0.9998. The average recoveries and the relative standard deviation were 101.0%,更多还原