【作者】 刘忠慧；

【导师】 陈国宏； 储明星；

【作者基本信息】 扬州大学 ， 动物遗传育种与繁殖， 2006， 硕士

【摘要】 本研究以200头山东省嘉祥种羊场小尾寒羊母羊和30头北京市顺义区杨镇种羊场多赛特羊为研究素材,选用绵羊促性腺激素释放激素受体(gonadotropin releasing hormone receptor, GnRHR)基因、骨形态发生蛋白15 ( bone morphogenetic protein 15, BMP15)基因、骨形态发生蛋白受体IB (bone morphogenetic protein IB receptor, BMPR-IB)基因多态性为研究内容,采用PCR-SSCP技术分析了GnRHR基因外显子2和外显子1部分序列在小尾寒羊、多赛特羊2个绵羊品种中的多态性,采用PCR-RFLP技术检测BMPR-IB基因和BMP15基因在高繁殖力绵羊品种小尾寒羊中的单核苷酸多态性,同时研究这两个基因对小尾寒羊高繁殖力的协同效应,旨在探索三个候选基因与小尾寒羊高繁殖力的关系。得到如下结论:更多还原

【Abstract】 Small Tail Han Sheep (200) comes from ShangDong provice and Dorset sheep(30) comes from BeiJing are studied as experimental material, Polymorphisms of Gonadotropin releasing hormone receptor(GnRHR) gene, Bone morphogenetic protein 15(BMP15)gene and Bone morphogenetic protein receptor (BMPR-IB) gene are detected, The polymorphism of exon 2 and part sequence of exon 1 of GnRHR gene in Small Tail Han sheep and Dorset sheep is analyzed by PCR-SSCP. Single nucleotide polymorphism of BMPR-IB and BMP15 genes are detected in Small Tail Han ewes by PCR-RFLP. The synergistic effect of the two genes on the prolificacy in Small Tail Han sheep is studied. We attempt to find the link of high productivity and those candidate genes,to provide some scientific evidence for our country’s basic genetic research on high productivity of small tail han sheep. The results are as the following: (1) The optimum method of PCR-SSCP about GnRHR gene has been established.更多还原