【作者】 任湘莲；

【导师】 杨章平； 常洪；

【作者基本信息】 扬州大学 ， 动物遗传育种与繁殖， 2006， 硕士

【摘要】 本研究是以中心产区典型群随机抽样法在湖羊中心产区—江苏省苏州市、滩羊的中心产区—宁夏回族自治区盐池县和乌珠穆沁羊中心产区—内蒙古自治区锡林郭勒盟东西乌珠穆沁旗分别抽取了具有独立血统的61头湖羊、60头滩羊和123头乌珠穆沁羊。在实验室采用PCR-SSCP方法研究了FSHR基因第10外显子、GDF9基因第1外显子在上述三个绵羊品种中的多态性;同时,选择与Booroola羊高繁殖力主效基因FecB紧密连锁的两个微卫星标记OarAE101和BM1329,分析其在上述三个绵羊品种中的多态分布情况,以寻找与绵羊繁殖性状相关的遗传标记,为绵羊高繁殖力的标记辅助选择和育种提供理论依据。结果如下:1.在FSHR基因第10外显子第1387～1617bp处设计引物,进行PCR-SSCP分析,建立了最佳PCR-SSCP条件,对61头湖羊、60头滩羊和123头乌珠穆沁羊进行了检测,结果只存在AA型个体。2.对FSHR基因第10外显子获取了PCR产物,首次测定了乌珠穆沁羊FSHR基因第10外显子第1425～1624bp处的序列。3.在GDF9基因第1外显子处设计两对引物:P1、P2,进行PCR-SSCP检测,结果发现P1的PCR-SSCP产物只有AA型个体;P2的PCR-SSCP产物有AA型和一个AB型个体,但PCR产物测序结果显示:AA型和AB型未有碱基改变。4.对与FecB基因紧密连锁的微卫星标记OarAE101和BM1329进行了检测,微卫星标记OarAE101在3个绵羊品种湖羊、滩羊、乌珠穆沁羊中的等位基因数分别为10、11和12;BM1329的等位基因数分别为8、5和8。微卫星标记OarAE101/BM1329在湖羊、滩羊、乌珠穆沁羊中的多态信息含量分别为更多还原

【Abstract】 Using the method of“random sampling in typical colonies of the central area of the habitat”, 61 Hu sheep,60 Tan sheep and 123 Ujumqin sheep were sampled respectively in Suzhou, Jiangsu Province; Ninxia Hui Nationality municipality and Ujumqin, Mogolia municipality. Using the method of PCR-SSCP and microsatelliate techniques to detect the variations in three sheep breeds above. The results indiacate that:1. The polymorphism of FSHR gene was analyzed in Hu sheep, Tan sheep and Ujumqin sheep, the result indicated that there was no mutation .2. The 1387～1587bp of exon 10 of FSHR gene was firstly sequenced in Ujumqin sheep.3. Two primers were designed to detect the polymorphism of GDF9 gene in three sheep breeds above. For primer 1, only AA genotype was detected. For primer 2, AA and AB genotype was observed, but the result of sequencing indicated that there were no mutation.4. OarAE101 and BM1329 were examined by the method of microsatelliate techniques. The numbers of allele gene(Ne) of OarAE101/BM1329 were 10/8,11/5 and 12/8 , respectively. The average polymorphism information contents(PIC) of OarAE101/BM1329 were separately 0.8961/0.7127, 0.8760/0.7565 and 0.8253/0.6667. The heterozygosities(h) of OarAE101/BM1329 valued 0.9010/0.7484, 0.8867/0.7832 and 0.8405/0.7120 , respectively。5. The detection of OarAE101 and BM1329 indicated that the PCR products of OarAE101 were all higher than 113 bp, and this may be the difference between higher fecundity sheep and lower fecundity sheep. The examination of BM1329 showed that there was no obvious diversity among the three sheep populations更多还原