【作者】 焦彩兰；

【导师】 王金玉； 储明星；

【作者基本信息】 扬州大学 ， 动物遗传育种与繁殖， 2006， 硕士

【摘要】 济宁青山羊是我国独特的地方山羊品种,具有较高的产羔数。BMP15基因影响Inverdale绵羊, Hanna绵羊, Lacaune绵羊, Belclare绵羊, Cambridge绵羊和小尾寒羊的产羔数。本研究以BMP15基因的多态性为研究内容,旨在探索该候选基因与济宁青山羊高产羔数之间的关系,为我国开展济宁青山羊高产羔数的遗传学基础研究提供科学依据。采用PCR-RFLP技术检测BMP15基因限制片断长度多态性。结果表明:在高产羔数山羊(济宁青山羊)和低产羔数的3个山羊品种(辽宁绒山羊、内蒙古绒山羊和波尔山羊)中都没有检测到BMP15基因的V31D突变(C→T),也没有检测到BMP15基因的Q23Ter突变(T→A)。排除了BMP15 V31D突变和Q23Ter突变影响济宁青山羊高产羔数的可能性。根据绵羊的BMP15基因序列,设计6对引物,采用PCR-SSCP技术检测BMP15基因2个外显子在高产羔数山羊品种(济宁青山羊)以及低产羔数山羊品种(辽宁绒山羊,内蒙古绒山羊和波尔山羊)中的单核苷酸多态性,同时研究该基因对济宁青山羊高产羔数的影响。结果表明:只有第五对引物扩增的片段检测到多态。在济宁青山羊中检测到AA、AB两种基因型,而在低产羔数的3个山羊品种中只检测到一种基因型AA。济宁青山羊AA基因型频率为0.10,AB基因型频率为0.90。克隆测序发现,BB型与AA型相比有2处核苷酸发生了突变(在DNA的第963bp处发生G→A突变;在DNA的第1050bp处发生G→C突变)。导致了2处氨基酸更多还原

【Abstract】 Jining Blue goat with high prolificacy is an exceptional local goat breed in China. Bone morphogenetic protein 15 (BMP15) gene which controls fecundity of Inverdale, Hanna, Lacaune, Belclare, Cambridge and Small Tailed Han ewes was studied as a candidate gene for the prolificacy of Jining Blue goats, to provide scientific evidence for the basic genetic research on high productivity of Jining Blue goat.Both V31D mutation in Inverdale ewes and Q23Ter mutation in Hanna ewes of BMP15 gene were not detected in Jining Blue goat, Liaoning Cashmere goat, Inner Mongolian goat and Boer goat by PCR-RFLP. The results indicated that maybe the fecundity of Jining Blue goat was not affected by both V31D mutation and Q23Ter mutation of BMP15 gene,.According to the sequence of ovine BMP15 gene, six pairs of primer were designed to detect single nucleotide polymorphism in exon 1 and exon 2 of the BMP15 gene in both high fecundity breed (Jining Blue goat) and low fecundity breeds (Liaoning Cashmere goat, Inner Mongolia Cashmere goat, Boer goat) by PCR-SSCP. Only the products amplified by primer F5/R5 displayed polymorphism. Results indicated that two genotypes (AA, AB) were detected in prolific Jining Blue goats, and only one genotype (AA) was detected in low fecundity goat breeds. In Jining Blue goats, frequency of genotype AA and AB was 0.10 and 0.90, respectively. Sequencing revealed two point mutations (G963A and G1050C) of BMP15 gene in the AB genotype in comparison to the AA genotype. The genotype distribution was significantly different between prolific Jining Blue goats and low fecundity goat breeds (P<0.001). There was no difference in genotype distribution between low fecundity goat breeds. The Jining更多还原