【作者】 郑敏；

【导师】 郭玲玲； 张庆慧；

【作者基本信息】 山东大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 【目的】：检测子宫内膜增生（endometrial hyperplasia）和子宫内膜样癌（endometrioid adenocarcinoma）组织中细胞周期调控蛋白S期激酶相关蛋白（S-phase kinase associated protein 2）SKP2和P27^Kipl的表达水平及正常子宫内膜和子宫内膜样癌组织中SKP2mRNA的表达情况。研究P27^Kipl和SKP2蛋白在子宫内膜增生和子宫内膜样癌中表达的生物学意义和临床价值，分析二者表达的相关性。探讨细胞周期调节失调与子宫内膜增生和子宫内膜样癌发生发展的关系。 【方法】：采用免疫组织化学PV9000二步法检测SKP2、P27^Kipl蛋白在10例正常分泌期子宫内膜、43例子宫内膜增生（单纯性增生13例、复杂性增生12例、不典型增生18例）和34例子宫内膜样癌中的表达水平；采用RT-PCR技术，对其中12例肿瘤组织和正常子宫内膜组织进行SKP2mRNA表达的检测，并结合临床病理学指标进行分析。 【结果】： 1．SKP2mRNA的表达情况：SKP2mRNA在子宫内膜样癌及正常子宫内膜组织中阳性率分别是75.00％和25.00％，二者的表达有显著性差异（P＜0.05）。 2．SKP2蛋白表达情况：在子宫内膜样癌中，SKP2呈过表达。其在正常子宫内膜、子宫内膜增生及子宫内膜样癌中的阳性表达率分别是0、39.53％、76.47％，SKP2蛋白在子宫内膜不同病变中的表达均有显著性差异（P＜0.05）。SKP2蛋白在内膜复杂性增生、中度不典型增生和重度不典型增生中的阳性表达率分别是33.33％、50.00％、100.00％，子宫内膜复杂性增生、中度不典型增生与重度不典型增生相比，SKP2更多还原

【Abstract】 Objective: To detect the expression level of cyclin-dependent kinase inhibitor, P27^Kip1 and S-phase kinase associated protein 2, SKP2 in endometrial hyperplasia and endometrioid adenocarcinoma. The expression of SKP2 mRNA was examined by RT-PCR in paraffin embedded carcinoma tissue and normal endometrial tissue.And the experssion of SKP2 and P27^Kip1 in the biological and clinical significance was discussed in endometrial hyperplasia and carcinoma. Aproach the relationship between the expression of SKP2 and P27^Kip1, then try to analysis the relationship between deregulation of the cell cycle in the occurrence and development of endometrial tumors. Methods: Immunohistochemistry was performed in the 10 normal endometrial tissue and 43 endometrial hyperplasia and 34 endometrioid adenocarcinoma to evaluate the expression level of the SKP2 and P27^Kip1 proteins.RT-PCR was performed in paraffin embedded normal endometrial tissue and endometrioid adenocarcinoma to detect mRNA expression of SKP2 gene. Result:1. RT-PCR analysis showed that SKP2 mRNA was overexpressed in endometrioid adenocarcinoma tissue, and the expression rate of SKP2 protein was raised in endometrioid adenocarcinoma tissue. The expression of SKP2 protein in normal endometrial gladular epithelium, endometrial hyperplasia and endometrioid更多还原