【作者】 郑轲；

【导师】 张克英；

【作者基本信息】 四川农业大学 ， 动物营养与饲料科学， 2006， 硕士

【摘要】 本文研究脲酶抑制剂和脲酶卵黄抗体对正常或应激条件下肉鸡腹水症发生的影响。试验选用288只1日龄艾维茵肉鸡为试验对象，随机分为8个处理组，在正常和应激（1.5mg／kgT₃）条件下，比较基础饲粮（对照组）、基础饲粮+120mg／kg脲酶抑制剂、基础饲粮+100mg／kg脲酶卵黄粉、基础饲粮+200mg／kg脲酶卵黄粉四种饲粮的效果，每个处理设8个重复，每个重复6只鸡，进行为期42天的饲养试验，测定21和42日龄的心脏指数、腹水症患病率、死亡率、日采食量、日增重、料重比、血氨、肠氨、黏膜形态和肠道菌群等指标。结果表明：（1） 正常条件下，饲粮中添加120mg／kg脲酶抑制剂增加42日龄体重、21-42和0-42日龄饲料转化率和42日龄双歧杆菌数。应激条件下，脲酶抑制剂减少21、42日龄的心脏指数、腹水症死亡率；降低21和42日龄血氨和大、小肠氨水平，显著减少42日龄大肠氨水平（P＜0.05）；改善21和42日龄空、回肠黏膜形态，显著增加21日龄空肠绒毛高度（P＜0.05），极显著降低21日龄回肠腺窝深度（P＜0.01）；改善21和42日龄盲肠微生物数量；增加21和42日龄体重、0-21日龄增重和各阶段的饲料转化率。（2） 正常条件下，饲粮中添加200mg／kg卵黄粉能提高21日龄体重，减少21日龄大肠氨水平，增加42日龄回肠绒毛高度，降低21日龄肠和42日龄空肠肌层厚度，提高42日龄双歧杆菌数，降低42日龄大肠杆菌数。应激条件下，200mg／kg卵黄粉增加21日龄体重、增重，21-42和0-42日龄饲料转化率；降低21和42日龄心脏指数和腹水症患病率、死亡率；减少21和42日龄血氨和大、小肠氨水平，显著降低42日龄鸡大肠氨浓度（P＜0.05）：改善21和42日龄空、回肠黏膜形态，显著增加21日龄空肠绒毛高度（P＜0.05），显著降低21日龄回肠腺窝深度（P＜0.05）；改善21和42日龄鸡盲肠微生物数量。（3） 正常条件下，100mg／kg脲酶卵黄粉增加21和42日龄体重，提高42日龄鸡双歧杆菌数量。应激条件下，100mg／kg脲酶卵黄粉提高0-21和0-42日龄饲料转化率；降低42日龄心脏指数和腹水症患病率、0-21和0-42日龄腹水症死亡率；降低21日龄回肠腺窝深度和肌层厚度，改善42日龄空肠形态，增加42日龄时的回肠绒毛高度，降低腺窝深度；增加21日龄乳酸杆菌，对42日龄鸡盲肠微生物也有改善。综上所述：120mg／kg脲酶抑制剂和200mg／kg脲酶卵黄抗体对应激条件下肉鸡腹水症患病有减轻趋势。更多还原

【Abstract】 A 6-wk trial was conducted to study the effects of urease inhibitor and anti-urease immunoglobulin Y on ascites in broilers under normal or stress condition with dietary T₃ （1.5mg/kg）. Two hundred and eighty-eight 1 day-old broiler were divided randomly into 8 treatments with 6 replicates of 6 broilers. Birds were assigned to a control or diets containing 120mg/kg urease inhibitor, or 100 or 200mg /kg anti-urease immunoglobulin Y in normal diet or diet with the addition of T₃ .The heart index,incidence and mortality of ascites, performance, ammonia in plasma, intestinal, intestinal morphology and intestinal flora was determined. The results were as follows:（1） With the addition of 120mg/kg urease inhibitor under normal condition,42d ABW, F/G of 21-42 and 1-42 d were improved;B.bacterium of cecm at day 42 was increased compared to the control group. Under stress condition, AHI and incidence of ascites at day 21 and 42, mortality of ascites at 42 d were lowered compared to the control group.The levels of metabolic ammonia at day 21 and 42 were decreased, the large intestine ammonia at 42d were lowered significantly（P<0.05）.The height of villus of ileum at day 21 and 42 were increased significantly（P<0.05）,the depth of crypt of ileum at 21d were lowered very significantly（P<0.01）.The intestinal flora at day 21 and 42 were improved,E.coli of cecum at 21 d were decreased（P<0.05）.The ABW at day 21 and 42,F/G at 1-21 d、 21-42d or 1- 42d were increased.（2）With the addition of 200mg/kg egg powder under normal condition, 21d ABW, the height of villus of ileum at day 42,B.bacterium in the cecum at 42d were improved. Large intestine ammonia at day 21, muscularis thickness of ileum at 21d and jejunum at day 42,E.coil in the cecum at day 42 were decreased compared to the control group.更多还原