【作者】 李荣；

【导师】 李俊；

【作者基本信息】 安徽医科大学 ， 药理学， 2006， 硕士

【摘要】 目的：本实验采用多种急、慢性炎症和免疫炎症性模型，并通过一系列体内、体外免疫药理实验，研究橙皮苷（hesperidin，HDN）抗炎免疫作用并探讨其部分作用机制。结果如下： 方法和结果： 1．HDN的抗炎作用： 采用二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足爪肿胀、棉球诱导的大鼠肉芽肿和佛氏完全佐剂（Freund’s complete adjuvant，FCA）诱导的佐剂性关节炎（adjuvant arthritis，AA）模型，观察HDN对实验动物炎症反应的抑制作用。 结果可见，HDN(120，240mg·kg^-1，ig)对二甲苯诱导的小鼠耳肿胀有明显的抑制作用；HDN(40，80，160mg·kg^-1，ig)对角叉菜胶诱导的大鼠足肿胀、棉球诱导的大鼠肉芽肿和AA大鼠的原发性炎症反应有明显的抑制作用。提示HDN具有明显的抗炎作用。 2．HDN的免疫调节作用 采用腹腔注射环磷酰胺复制免疫功能低下的动物模型；测定胸腺、脾脏重量计算脏器指数；碳廓清法测定单核巨噬细胞吞噬功能；分光光度法测定血清溶血素HC_IgM、HC_IgG；QHS法测定分泌细胞溶血空斑值（PFC）；MTT法测定脾淋巴细胞增殖反应；以二硝基氟苯（dinitrofluorobenzene，DNFB）诱导迟发型变态反应（delayed-type hypersensitivity，DTH）模型，观察HDN对小鼠DTH和T淋巴细胞亚群的影响。 结果可见，HDN(120，240mg·kg^-1，ig)可显著提高免疫低下小鼠的脏器指数、碳廓清指数K和吞噬指数α，提高免疫低下小鼠的巨噬细胞吞噬功能，HDN(60，120，240mg·kg^-1，ig)可以上调免疫抑制小鼠低下的脾淋巴细胞增殖反应：HDN(120，240mg·kg^-1，ig)恢复免疫抑制小鼠DTH，各个剂量组的HDN均可以提更多还原

【Abstract】 AIM:The acute, chronic inflammatory and immune inflammatory （adjuvant arthritis, AA） models were established in order to investigate the anti-inflammatory and immunomodulatory effects of hesperidin and its mechanism, meanwhile a series of immumopharmacological experiments in vivo and in vitro were adopted. METHODS AND RESULTS:1. Anti-inflammatory actions of HDNFour inflammatory models were used to investigate the anti-inflammatory actions of HDN, which were dimethylbenzene-induced mouse ear edema, carrageenan-induced rat hind paws swelling, cotton-induced mouse granuloma and FCA-induced AA models.HDN could remarkably inhibit the ear edema induced by dimethylbenzene in mice at the dose of 120,240 mg·kg^-1, and reduce the swelling of rats hind paws induced by carrageenan, the weight of granuloma induced by cotton in rats, primary inflammatory reaction in AA rats at the dose of 40,80,160 mg-kg"1 respectively. The results showed HDN have a remarkable anti-inflammatory action.2. Immunoregulatory actions of HDNThe immunosuppressed mice were induced by cyclophosphamide （Cy, ip.）. Index of immune organs was weighed and calculated. Phagocytosis of mononuclear macrophage was determined by cleaning carbon particle method. Spectrophotography was used to estimate levels of serum hemolysis IgG, IgM. Plaque forming cell were determined with QHS. Splenocyte lymphocytes proliferation was measured by MTT assay. The mouse DTH model induced by DNFB was used to study the effects of hesperidin on the levels of DTH and subset of T lymphocyte.更多还原