【作者】 赵明；

【导师】 周建伟；

【作者基本信息】 南京医科大学 ， 劳动卫生与环境卫生学， 2006， 硕士

【摘要】 人类疾病往往是环境因素与遗传因素共同作用的结果，探讨基因与环境的相互作用机制与规律，对于防治环境因素造成的机体危害具有重要意义。 1998年，周建伟等利用差异显示高通量筛选技术从培养的原代人的气管和支气管上皮细胞中分离克隆了一个在生物进化上高度保守的环境应答基因-JWA，其编码蛋白是一种新的微管相关蛋白，并受多种分化诱导剂如氯化高铁血红素(hemin)等调控。该基因可能参与多种白血病细胞的定向分化和凋亡，并与细胞应答环境中的热应激的信号转导通路有关。 为了进一步深入了解JWA基因与红白血病的关系，本课题用hemin、热应激及hemin与热应激联合处理K562细胞，观察JWA基因在这些细胞模型中表达的特点和规律，探讨JWA在细胞应答hemin和应激信号通路中的作用和分子机制，以期为发展新的物理疗法与化疗结合治疗红白血病提供依据。 一、JWA对hemin处理K562细胞的应答：更多还原

【Abstract】 Human diseases are usually the consequences of the interactions between environmental agents and genetic. The present study is focus on the behind mechanisms of JWA gene in responding the stimulations of both hemin and thermal stress in K562 cells.In 1998, Zhou et al cloned a novel environment-responsive gene from human bronchial epithelial (HBE) cells by using the differential display high-throughput technique (AF070523). The new gene, named JWA, is broadly distributed in most of examined eukaryotic cells and tissues. The intracellular distribution of JWA protein encoded by the gene is very similar to that of cytoskeleton under immunofluorescence microscopy. The gene expressions are modulated by some cell differentiation inducers, like hemin. Our recent studies showed that JWA is also a functional protein associated with cell differentiation and apoptosis, and might play an important role in development更多还原