【作者】 陈凤凰；

【导师】 唐文明；

【作者基本信息】 贵州大学 ， 有机化学， 2006， 硕士

【摘要】 对中药半夏（Pinellia ternata（Thunb.）Breit）的干燥块茎、核桃（Juglans regia）树皮的化学成分进行了分析。通过溶剂提取，硅胶柱层析、聚酰胺柱层析、制备薄层层析、重结晶等进行分离。从半夏中分离得到12个化合物，经过IR、¹H-NMR、EI-MS等波谱分析，鉴定了10个化合物，分别为β-谷甾醇、胡萝卜苷、琥珀酸、4-氨基丁酸、丙氨酸、棕榈酸、棕榈酰胺、正三十一烷、正三十酸、2-羟基二十六酸。其中化合物4-氨基丁酸、丙氨酸为首次从半夏中分离得到，化合物棕榈酰胺为首次在半夏中发现和分离。从核桃树皮中分离得到7个化合物，目前通过IR、¹H-NMR、¹³C-NMR、EI-MS等波谱分析，鉴定了3个化合物，分别为正三十酸、没食子酸、β-谷甾醇。其它4个，其中有一个是蒽醌类化合物、一个白桦脂醇衍生物，一个芳香族化合物。 将核桃树皮粗提取液以及从半夏、核桃树皮中分离得到的部分化合物进行生物活性测试。结果表明，核桃树皮粗提取液在0.2 mg／mL时，对人白血病细胞株K562、人非小细胞肺癌细胞株A549有很强的抑制作用。化合物没食子酸在浓度为0.02mg／mL时，对人白血病细胞株K562有64.78％的抑制率。化合物HT-12在浓度约为1×10^-5 mol／L时，对K562、A549分别有88.32％、65.77％的抑制率，具有较好的活性，从而推测HT-12可能是核桃树皮抗癌作用的有效成分之一。更多还原

【Abstract】 The chemical constituents of the dried tubers of Pinella ternate （Thunb.）Breit and the bark of Juglans regia were researched. Twelve compounds were separated by column chromatography on silica and purified by recrystallization, preparative TLC on silica gel GF254 from the ethanol extracts of P. ternate. Ten compounds were identified as β-sitosterol, daucosterol, succinic acid, 4-aminobutyric acid, alanine, palmitic acid, palmitic amide, hentriacontane, triacontanoic acid, 2-hydroxyhexacosanoic acid by spectroscopic analysis, such as IR, ¹H-NMR, EI-MS. Alanine, 4-aminobutyric acid were isolated from this plant for the first time. Palmitic amide was found and isolated from this plant for the first time.Seven compounds were isolated from the bark of Juglans regia by column chromatography on silica and polyamide gel. Three compounds were determined as triacontanoic acid, gallic acid,β-sitosterol by IR, ¹H-NMR, ¹³C-NMR, EI-MS methods.The biologically activity of partial compounds which isolated from two plants and crude extracts of the bark of Juglans regia. The result is that crude extracts of the bark of Juglans regia possess better activities against K562 and A549 （ at 0.2 mg/mL ）. Gallic acid against KS62 （64.78% at 0.02 mg/mL） . Compound HT-12 against K562 and A549 (88.32% and 65.77% at 1×10^-5 mol/L respectively) . It possesses significant anticancer activity. Compound HT-12 may be one of anticancer constituents of the bark of Juglans regia.更多还原