【作者】 侯殿东；

【导师】 刘辉；

【作者基本信息】 大连医科大学 ， 免疫学， 2006， 硕士

【摘要】 研究表明应激能够影响动物和人体的免疫功能,机体免疫系统功能的紊乱是感染性疾病、肿瘤等发生或恶化的直接原因,故阐明应激对免疫系统损伤的作用机制具有重要意义。动物模型具有资源相对易获得、操作性强、涉及伦理道德问题相对较少等优点,因此建立理想的应激动物模型是研究得以深入的关键。我们应用束缚应激和热应激建立了两种小鼠应激动物模型,然后使小鼠染菌,通过比较应激状态下小鼠与正常小鼠的死亡率来研究两种应激对小鼠免疫防御功能的影响,并试图从遗传背景角度揭示基因多态性与应激状态下免疫应答强度的关系;通过应激所导致的机体免疫器官损伤、血清中蛋白质的变化及全血中金属元素含量的变化几个方面来揭示应激对免疫防御功能影响的相关机制。方法应用束缚应激和热应激建立了两种小鼠应激动物模型,然后使小鼠染菌,研究这两种应激对小鼠免疫防御功能影响,并通过以下几个方面来研究应激对免疫功能损伤的可能机制。1.研究应激对小鼠脾脏重量指数的影响,观察接受应激的小鼠脾脏的病理变化,阐述免疫防御功能降低的构效关系。2.以已经建立的动物模型为研究对象,采用PCR技术检测以MudoEb5和MudoEb7为代表的小鼠H2-Eb位点等位基因的多态性,从遗传多样性的角度揭示基因多态性与应激状态下免疫应答强度的关系。3.采用SELDI-TOF-MS技术,运用弱阳离子交换芯片(WCX2)对小鼠血清蛋白质指纹图谱进行研究,探索不同类型的应激对免疫功能影响的特点及物质基础。更多还原

【Abstract】 BackgroundIt has been proved that stress could modify multiple aspects of immune responses. Immune imbalance is the contributory factor to the onset or progression of infectious, autoimmune, and malignant diseases, so it is important to illuminate the mechanisms of impaired immune response under stress. There are many virtues in animal model for scientific research, therefore establishing successful animal modes is the key for further research in stress field. Two kinds of stressed mice models (restraint stress and heat stress) were established in the test, then the mice were attacked by Escherichia coli, the mortality rate of mice of each group was calculated, the influences on the immune defense of mice under different stressors were explored. The relations between genetic polymorphisms and the immune response under stressors were investigated. And the related mechanisms were studied through the researches of damages of the immune apparatus, changes of the proteins in the serum, and alterations of the contents of tantalum in the whole blood under stressors.MethodsTwo kinds of stressed mice models (restraint stress and heat stress) were established, then the mice were attacked by Escherichia coli, the mortality rate of mice of each group was calculated, the influences on the immune defense in mice under different stressors were explored. And the related mechanisms of impaired immune response under stress were studied through the following tests.1. The spleen indexes of the mice of each group were compared, and routine pathological technique and morphometric measurements were used to observe更多还原