【作者】 姚文艳；

【导师】 雄鹰；

【作者基本信息】 大连医科大学 ， 神经内科， 2006， 硕士

【摘要】 目的:经导管栓塞术是介入治疗中的重要技术。它已广泛应用神经科疾病的治疗中,如脑动静脉瘘、脑动静脉畸形、动脉瘤、脑富血运肿瘤。它的成功应用有三个重要条件:适合的栓塞剂、严格的适应症和熟练的操作技术。因此栓塞材料的不断完善对提高血管内栓塞治疗的水平有重要意义。目前的栓塞材料按照其物理性质分为固体和液体两种,固体栓塞剂包括机械栓塞材料和颗粒栓塞剂。颗粒栓塞剂是最早被应用于临床的栓塞材料。但是因为其形态多不规则,大小不均,透视下不能分辨等缺点限制了其应用范围。本试验将碘油包埋入海藻酸钠微球使它具有在透视下可以显影的功能,并通过调整制备条件得到不同粒径的栓塞剂。减少了术中操作的复杂性及盲目性,可以适应不同的栓塞需要。方法:1.采用静电液滴法制备海藻酸钠微球。观察微球的形态,大小。2.改变电压、频率、液体流量、喷嘴内径和溶液浓度等制备条件,制备海藻酸钠微球,用CV表征微球单分散性和平均粒径表征粒径的大小。3.将1.5%海藻酸钠溶液和碘化油分别乳化后,混合并充分搅拌,用静电液滴法制备含碘的海藻酸钠微球,碘油含量分别为5%、10%、15%、20%、25%。在显微镜下和X-ray下观察微球的形态和显影效果。4.使用含碘油20%的海藻酸钠微球栓塞家兔右侧颈总动脉。观察栓塞后动物行为和功能的变化,定期行影像学和病理学检查。结果:1.静电液滴法制备出的海藻酸钠微球形态完整、大小均匀、表面光滑。2.电压和频率对单分散胶珠的平均粒径影响不大。随着溶液的浓度、流量和喷嘴内径的增大,胶珠的粒径呈增大趋势。3.含碘海藻酸钠微球呈白色,表面光滑,无粘连,不堵管,在x-ray下显影清晰。随着碘油含量的增高,显影逐渐清晰。4.动物于术后无明显不良反应。该栓塞剂在体内更多还原

【Abstract】 Objective: Transcatheter Arterial Embolization (TCAE) is one of the three techniques in interventional treatment. It is widely used in the treatment of neural system diseases, such as aneurysm, arteriovascular malformation and meningiomas. A successful application of TCAE must have three elements: appropriate embolic materials, strict indications and proficient manipulation. So the development of embolic materials is very important. The granule is the earlist embolic materials . But most of them are radiolucent, irregular on the surface and diameter .So that the usage of them is limited. In this study we prepared a radiopaque embolic microspheres-- Iodine-loaded alginate, which can eliminate the complexity and blindness during the embolization. And the microspheres can be calibrated, we can choose microspheres with different diameters according to the disease.Methods: 1. Prepared calcium alginate microspheres in high voltage electrostatic field. Observed the shape and the size of the microspheres.2. Prepared calcium alginate microspheres in different applied potential、frequency and、flow rate of solution through the capillary、the diameter of the nozzle and the concentration of alginate solution .Estimated the monodispersity and diameterso the microspheres by CV and mean diameter. 3. Mix the 1.5% alginate solution and iodine oil after they are emulsified separately. Then prepared iodine-loaded alginate microspheres in high voltage electrostatic field. The content of iodine oil is from 5%、10%、15%、20% to 25%。Observed their shape under microscope , their development under X-ray were judged by X-ray photos.4. Embolize right更多还原