蒙药娜仁满都拉-11对荷瘤大鼠IL-4和IFN-γ调节作用的研究

【作者】 王海生；

【导师】 刘淑萍；

【作者基本信息】 内蒙古医学院 ， 免疫学， 2006， 硕士

【摘要】 目的 建立Walker-256癌肉瘤腹水型及种植性胃肿瘤大鼠实验模型，研究蒙药娜仁满都拉-11的抗肿瘤作用及其对IL-4、IFN-γ的调节作用，为进一步开发蒙药娜仁满都拉-11提供实验依据。方法 利用Walker-256癌肉瘤细胞株，建立腹水型及种植性胃肿瘤大鼠模型，将荷瘤鼠随机分为治疗组和对照组，治疗组给予不同剂量娜仁满都拉-11灌胃，阴性对照组用生理盐水灌胃，阳性对照组用5-Fu灌胃。10天后采集实验鼠心脏血，并取出瘤组织称重。从心脏血中分离单个核细胞进行细胞培养，用ELISA和RT-PCR法分别检测IL-4、IFN-γmRNA的含量。结果 (1)成功建立Walker-256癌肉瘤腹水型及种植性胃肿瘤大鼠实验模型；(2)蒙药娜仁满都拉能显著改善肿瘤鼠生存状态，对胃肿瘤具有一定的抑制作用，瘤重抑制率为44％；(3)蒙药娜仁满都拉-11治疗组动物IL-4水平显著低于阴性对照组，而IFN-γmRNA水平显著高于阴性对照组。结论 蒙药娜仁满都拉-11对Walker-256癌肉瘤腹水型及种植性胃肿瘤大鼠具有一定的抗肿瘤作用，并具有一定的免疫调节作用，可增强IFN-γmRNA的表达，降低IL-4的表达。更多还原

【Abstract】 Objective Duplicated walker - 256 ascite model and implanted tumor of Walker - 256 cells on the animal model, study the anti - tumor effect of Na Ren Man Du La - 11 and influence on immune adjustment on IL - 4、IFN-γ, provide evidence for the further study of developing Na Ren Man Du La - 11. Method Walker - 256 cell lines were used to duplicate ascite model and implanted tumor model in mice, the mice bearing tumor were divided into treatment group and control group, different dose of Na Ren Man Du La- 11 were given to the mice in treatment group, 0. 9%NaCl to those in negative control group and 5 - Fu to those in positive control group. 10 days later we collected the heart blood, get out tumor tissue and test their weights, then separate mononuclear cell from heart blood and cultivated them. ELISA and RT - PCR were used to test quantity the expression of IL - 4 and IFN -γmRNA. Results (1) Ascite model and implanted gastric tumor model in mice were duplicated successfully by using malignant cell line Walker - 256. (2) Na Ren Man Du La - 11 can obviously improve the life qualities of mice. Implanted gastric tumor can be inhibited in some degree. Weight inhibit rate is 44% . (3) IL - 4 is lower in treatment group than in negative control group , while IFN - γmRNA is higher than in control group. Conclusion Na Ren Man Du La - 11 has anti - tumor function in treating ascite model and implanted tumor of Walker - 256 cells on the animal model, it also has function in immune adjustment by enhance the expression of IFN -γ mRNA and lower IL - 4 in ascite model.更多还原