【作者】 王惠国；

【导师】 王亚贤；

【作者基本信息】 黑龙江中医药大学 ， 中西医结合基础， 2006， 硕士

【摘要】 系统性红斑狼疮(systemic lupus erythematosus,SLE)是一种严重威胁人类健康的自身免疫性疾病,有较高的病死率和致残率,常发生于12-40岁的青壮年女性。 SLE的免疫病理特征是机体释放出大量细胞核内容物成为自身抗原,自身抗原与自身抗体结合形成免疫复合物,引发免疫复合物病,常累及肾、肝、心、脑、血液等重要组织器官。 目前临床尚无特别有效的系统性红斑狼疮治疗方法,中医药为主的中西医结合治疗SLE具有较好的前景。祛毒胶囊是我校附属医院皮肤科经验方,临床治疗系统性红斑狼疮取得了良好的效果。 本课题的目的在于研究祛毒胶囊对系统性红斑狼疮样小鼠的治疗作用,探讨祛毒胶囊治疗SLE的免疫调节机理。参照WHO免疫研究教学中心报道的方法造模,将75只BALB/c雌性小鼠随机分为5组:A正常对照组、B模型对照组、C祛毒胶囊治疗组、D醋酸泼尼松治疗组、E祛毒胶囊和醋酸泼尼松联合治疗组。选取与SLE关系密切的IL-10和抗ds-DNA抗体作为观测指标。第15天,用ELISA法测定各组小鼠血清中IL-10和抗ds-DNA抗体的水平,取出肝、肾组织做石蜡切片观察病理改变。 结果显示:造模后小鼠出现便溏、倦卧、食少等表现;模型组血清中IL-10和抗ds-DNA抗体水平比其他各组显著升高(p<0.01);病理结果显示模型鼠肝肾炎性改变。治疗后,用药各组小鼠一般状况均有不同程度的恢复,中药加激素组的一般状况改善最为明显;祛毒胶囊能降低血清中抗ds-DNA抗体及IL-10水平,有利于纠正SLE免疫紊乱;祛毒胶囊治疗组动物肝、肾炎性改变减轻。更多还原

【Abstract】 Systemic Lupus Erythematosus (SLE) is the Auto-immune disease with multi-system involvement.It is one of the difficult problems in medical field, and mortality is very high.Young women (12-40) suffer from this disease easily.Systemic lupus erythematosus (SLE) is characterized by the presence of autoantibodies in the patient serum. As an autoimmune disease involving many systems in the body.SLE inevitably results in many effects and injuries in the liver, kidney, heart, blood,system.It is one of the common diseases difficult and there is still no therapy remarkably effective for it. Combination of western and traditional medicine dominated by the traditional Chinese herbal medicines and accompanied with western medicine is acknowledged as an effective one owing to its remarkable effectiveness.QDJN sums up view in longtime treatment practice.The prevention and treatment of SLE achievements have been reported in recent years.Objective:To observe QDJN has good therapy effect, adjusting cytokine levels,helping to reducing effect on the disease activity of SLE mice.Methods: We selected BALB/c female mice,with age of 8~10weeks, Do as WHO (WHO IMMUNOLOGY RESEARCH AND TRAINING CENTRE) Tito Cavalo、 Francisco has reported method,i.p LPS 2.5mg/kg to repeat the SLE model.75 SLE mice were at random divided into 5 group.A group is acted as negative group;B is model group;C group is treated by QDJN;D group is treated with west medicine;E group is treated by combination of Western and traditional medicine.All groups were treated fifteen days.We examined the level of IL-10 and anti-ds-DNA change by ELISA method, and the effect of QDJN treating effects on liver,kidney.Result:IL-10 and ds-DNA level in A^ C> D> E groups were significantly lower than that in group B.The SLE mice injuries in the liver, kidney in group CDE decreased signifcantly compared with that of group B.In conclusion, our studies show firm evidence that QDJN can really treat SLE by decreasing the level of IL-10 and ds-DNA in SLE mice blood.QDJN can improve the injury of kidney and liver of SLE mice.更多还原