高压输电线电磁辐射对小鼠成纤维L929细胞增殖活性的影响

【作者】 刘乐维；

【导师】 庞小峰；

【作者基本信息】 电子科技大学 ， 生物物理学， 2006， 硕士

【摘要】 由于工业和农业的迅速发展,高压输电线布满了我们生活的空间。高压输电线电磁辐射对于人类的健康是否有影响及有何影响一直引起人们的关注。在本论文中,我们仔细的考查了高压输电线电磁辐射对离体小鼠成纤维细胞增殖活性的影响。本实验选用小鼠成纤维L929细胞株作为离体细胞模型,分别通过倒置相差显微镜观察法、噻唑蓝(MTT)比色分析法,细胞凋亡的DNA Ladder检测法、流式细胞术等技术检测了在0,20,40,60,80,100分钟的模拟高压输电线的电磁辐射(其电场和磁场强度分别为E=4000V/m,B=0.1G)暴露后对细胞形态、细胞增殖活力、细胞凋亡、细胞周期的影响。通过这些实验,我们得到:1.高压输电线电磁辐射暴露时间越长的L929细胞,分化速度越慢,而且个别细胞变小、变圆,单独生长,单位面积内细胞数、集落数显著减少。2.用不同时间的高压输电线的电磁辐射和该辐射单独的电场照射小鼠成纤维L929细胞,发现它们对细胞的增殖有显著的抑制作用(P<0.05),抑制程度随暴露时间的增加而增强,且电场抑制程度更强。若单独用该辐射的磁场照射细胞,发现细胞的增殖活性显著性增强(P<0.05),增殖活性随暴露时间的增加而增强。表明电场与磁场对L929细胞增殖有不同的效应。3.高压输电线电磁辐射在0-100分钟的照射时间内没有发现L929细胞有大量凋亡现象出现。4.高压输电线电磁辐射能使L929细胞阻滞于G1和G2 -M期,抑制细胞的增殖效应,而且呈一定的时效关系。通过这些结果说明,高压输电线电磁辐射能够改变L929细胞的形态,抑制L929细胞的增殖活性,而这种效应随照射时间的增长变得越来越明显。但高压输电线的电磁辐射中的电场在这种效应中起了主导作用。更多还原

【Abstract】 Since the rapid development of the industry and agriculture, the high voltage transmission lines (HVTL) are widespread in the envrionment. The health risks posed by electromagnetic fields of HVTL attract our attentions. In this thesis, the effects of electromagnetic fields of HVTL on the proliferation of fibroblast cell in vitro have been investigated.The experimental materials in this paper are mice fibroblast cell line L929. The effects of the electromagnetic fields of HVTL (f=50Hz, E=4000V/m, B=0.1G) exposure on morphology, cell proliferation, cell cycle and apoptosis are investigated by the methods of phase contrast microscope, MTT colorimetric assay, agarose gel electrophoresis and flow cytometry. The main results are as follows:1. The L929 represented the unremarkable colonies, low speed of growth and differentiation, reduced density as well as shrinking of synapsis along with time of the electromagnetic fields of HVTL exposure.2. The result of MTT colorimetric assay revealed that the proliferation of L929 exposed by the electromagnetic fields of HVTL and the electric fields could be significantly inhibited in comparison to the control group (P<0.05). On the contrary, exposed to the magnetic fields could significantly stimulate the proliferation of L929 (P<0.05). The result showed that the effects of the electric fields and the magnetic fields of HVTL on proliferation were different.3. After exposing to the electromagnetic fields of HVTL from 0 to 100 minute, apoptosis of L929 wasn’t observed through the agarose gel electrophoresis.4. FCM analysis showed that the L929 cell cycles of the electromagnetic fields of HVTL exposure groups were blocked in G0/1 and G2-M.In summary, these results demonstrated that the electromagnetic fields of HVTL exposure have effect on inhibition of proliferation and cell cycle of L929 but no effect on apoptosis. Meanwhile, the electric fields of HVTL play a chief role on above effects.更多还原