【作者】 杨继文；

【导师】 张秋才；

【作者基本信息】 河北医科大学 ， 中西医结合临床， 2006， 硕士

【摘要】 目的:脑出血是常见病、多发病,有较高的死亡率和致残率,尤其在急性期死亡率更高,目前无特效的治疗方法。补气活血法是继活血化瘀法之后中医治疗脑出血急性期的又一重要治疗方法,该法在临床治疗脑出血急性期显示较好的疗效,但该法对脑出血急性期干预机制的研究很少。本实验中,选用补气药黄芪注射液和活血化瘀药丹参粉针2种药物配伍组成补气活血法;丹参粉针代表活血化瘀法。通过建立大鼠脑出血模型,观察补气活血法对脑出血大鼠急性期神经行为学和组织学的影响,以及该法对大鼠血肿周围神经细胞凋亡、Bcl-2蛋白、核因子κB p65(NF-κB p65)和诱导型一氧化氮合酶(iNOS)表达的影响。并探讨该法对脑出血后病理损伤的干预机制,为临床治疗脑出血的方法提供理论上的依据。方法:实验用SD雄性大鼠104只,体重300～350g,由河北医科大学动物实验中心提供。随机分为正常组、假手术组、模型组、活血化瘀组、补气活血组,共5组。正常组8只;其余4组均为24只,按手术后处死时间又随机分别分为1天组、3天组、7天组3个小组,每小组均为8只。正常组大鼠自由饮食,不做特殊处理;模型组大鼠在麻醉后,断尾取血,在立体定向仪下注入尾壳核,术后自由饮食;假手术组只进针,不注血,其它操作同模型组;活血化瘀组在制成脑出血模型后,每日腹腔注射丹参粉针溶解液更多还原

【Abstract】 Objective: Intracerebral hemorrhage (ICH) is a severe type of acute cerebrovascular disease with high morbidity and mortality. There is not a very effective therapy after ICH. Invigorating vital energy and promoting blood flow therapy (IPT) is another therapy of ICH at the acute stage besides promoting blood flow and removing stasis therapy (PRT) of Traditional Chinese Medicine. IPT has a better therapeutic effect in clinic at the acute stage, but known little about the intervention mechanisms in treating ICH. In the experiment, IPT is made up of Astragalus injection and Salviae miltiorrhiza radix injection, PRT is made up of Salviae miltiorrhiza radix injection only. Injecting of autologous blood of rat made ICH model. The score of neural functional defect, microanatomy, apoptosis cells, the cells expressing protein of Bcl-2, NF-κB p65 and inducible nitric oxide synthase (iNOS) were measured after treatment by IPT. This paper may provid theoretical foundation that IPT prevents and treats the secondary injury of ICH for clinic.Methods: 104 Sprague-Dawley male rats (300-350g) were offered by Hebei Medical University. The rats were divided into normal group, sham group, model group, PRT group and IPT更多还原