【作者】 李涛；

【导师】 温浩； 赵晋明；

【作者基本信息】 新疆医科大学 ， 普外科， 2006， 硕士

【摘要】 目的：建立BN大鼠对感染泡球蚴LEW大鼠的原位肝脏移植模型(Orthotopic liver transplantation OLT)，探讨感染泡球蚴的大鼠肝移植后，其免疫系统的变化特点及其对移植物的影响。方法：采用两袖套法建立BN大鼠供肝移植到LEW大鼠的原位肝脏移植模型。实验选用96只健康的Brown norway(BN)雄性大鼠，96只Lewis(LEW)雄性大鼠，包括48只LEW健康大鼠和48只感染泡球蚴LEW大鼠。实验分为5组：1)A组，正常对照组(同系移植)：健康LEW大鼠→健康LEW大鼠；2)B组，对照组(非同系移植)：健康BN大鼠→健康LEW大鼠；3)C组，实验组：BN大鼠→LEW大鼠(感染泡球蚴后3个月)；4)D组，用药对照组：BN大鼠→健康LEW大鼠+他克莫司(FK506)灌胃，0.1mg／(kg·d)；5)E组，用药实验组：BN大鼠→LEW大鼠(感染泡球蚴后3个月)+他克莫司(FK506)灌胃，0.1mg／(kg·d)。术后LEW大鼠存活48小时判定为肝脏移植手术成功，48小时内死亡的LEW大鼠不纳入实验中并及时补足大鼠只数。每组于术后1，3，5，7天四个时间点各处死2只LEW大鼠，取血清、移植的肝脏、脾脏。每组留8只用于观察生存期。结果：A组、B组、C组、D组、E组受体存活时间平均分为54.13±16.62天、4.75±1.58天、15.50±3.93天、15.00±4.50天和15.75±3.06天。A、C、D和E组受体存活时间较B组明显延长，差异有统计学意义(p＜0.05)；D组和E组受体存活时间差异无统计学意义(p＞0.05)。病理结果示：1)A组和D组均无排斥反应现象；2)B组排斥反应随移植天数的增加更多还原

【Abstract】 Objectives: To investigate the immune characteristics of rats infected with Echinococcosus multicularis(E.m) and its effects on the graft by using BN-to-LEW orthotopic liver transplantation(OLT) models. Methods: Two-cuff method was adopted in the BN-to-LEW OLT models. 96 health BN and 96 LEW rats were used in the current study, the LEW rats consited of 48 health rats and 48 rats with E.m infection.The animals were randomly divided into five groups as follows: Group A normal controls were produced by (OLT in the same strain)from LEW-to- LEW; Group B.strain controls were produced by (OLT in the different strain)form BN-to-health LEW; Group C: E.m stimulated growp were produced by BN-to-LEW rat with E.m infection for 3 months; Group D: drug treated group were produced by BN-to-health LEW and Tacrolimus (FK506) after OLT(0.1mg/(kg·d)); Group E: E.m cambined drug treated group were produced by BN-to-LEW rat with Em infection for 3 months and Tacrolimus(FK506) after OLT(0.1mg/(kg·d)). The OLT procedure was considered as succesed model when the LEW rat can be survived over 48h. If any modle was died within 48h ,the rat was excluded in the study. The LEW ser??, liver graft, spleen samples were collected in 1,3,5,7 days after OLT respectively. Eight LEW rats in each group were remained for observation of their the survival days. Results: The mean survival times for each group were 54.13±16.62d for group A, 4.75±1.58d更多还原