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青海海西州传统发酵酸山羊奶的化学与微生物组成分析及乳酸菌的分离鉴定
Study on Chemical and Microbiological Compositions and Identification of Lactic Acid Bacteria from Natural Fermented Goat Milk in Qinghai
【作者】 云月英;
【导师】 张和平;
【作者基本信息】 内蒙古农业大学 , 食品科学, 2006, 硕士
【摘要】 本项研究对采集于青海海西州德令哈地区的11份酸山羊奶样品进行了化学和微生物组成分析,并对分离到的33株菌进行了属种的鉴定,结果表明:11份酸山羊奶样品的脂肪、蛋白质、乳糖、干物质、灰分平均含量为3.69±1.51g/100mL、5.75±0.87g/100mL、1.92±0.35g/100g、11.35±1.59g/ 100mL、0.95±0.05g/100mL;pH值平均为3.9,滴定酸度平均为194.9±19.6oT,采样温度平均为13.8℃; VC、VB1、VB2、VB6、乳酸、乙酸、柠檬酸、乙醇平均含量为1.359±0.79 g/100g、0.935±0.729μg/g、8.474±2.856μg/g、0.638±0.455μg/g、1.379±0.294g/100g、0.010±0.017 g/100g、0.217±0.053 g/100g、2.102±1.786g/L;其中10份样品的Ca、P、K、Mg、Na、Fe、Zn、S平均含量为2063.81±260.87mg/kg、1629.40±160.37 mg/kg、1776.31±251.21 mg/kg、194.19±24.48 mg/kg、371.37±55.33 mg/kg、2.46±1.11 mg/kg、4.47±0.85 mg/kg、601.25±67.18 mg/kg,Mn、Cu的含量均低于检测极限。乳酸菌数在2.49×108~2.98×109cfu/mL间;酵母菌数在2.60×106~1.63×108cfu/mL间。从11份酸山羊奶样品中分离到33株菌,15株杆菌,18株球菌。其中德氏乳杆菌德氏亚种(Lb. delbrueckii subsp. delbrueckii) 7株;德氏乳杆菌保加利亚亚种(Lb. dellbrueckii subsp. bulgaricus)4株;嗜酸乳杆菌(Lb. acidophilus)3株;棒状乳杆菌棒状亚种(Lb. coryniformis subsp. coryniformis)1株;嗜热链球菌(Streptococcus thermophilus)10株;乳酸片球菌(Ped. acidilactici)5株;粪肠球菌(Enterococcus faecalis)、鸟肠球菌(Enterococcus avium)、肠膜明串珠菌肠膜亚种(Leu. mesenteroides subsp. mesenteroides)各1株。其中Lb. delbrueckii subsp. delbrueckii和Streptococcus thermophilus为优势菌株。
【Abstract】 Chemical and microbiological compositions of 11 samples of natural fermented goat milk in Qinghai province were determined. The average contents of fat, protein, total solids and ash were 3.69±1.51, 5.75±0.87, 11.35±1.59 and 0.95±0.05g/100mL respectively, lactose was 1.92±0.35 g/100g. On average, pH was 3.9, titratableacidity was 194.9±19.6°T, temperature was 13.8℃. The average contents of VC, VB1, VB2, VB6, lactic acid, acetic acid, citric acid and ethanol were 1.359±0.79g/100g, 0.935±0.729μg/g, 8.474±2.856μg/g, 0.638±0.455μg/g, 1.379±0.294g/ 100g, 0.010±0.017 g/100g, 0.217±0.053 g/100g and 2.102±1.786g/L. The average contents of Ca, P, K, Mg, Na, Fe, Zn and S were 2063.81±260.87mg/kg、1629.40±160.37mg/kg、1776.31±251.21mg/kg、194.19±24.48mg/kg、371.37±55.33mg/kg、2.46±1.11 mg/kg、4.47±0.85 mg/kg、601.25±67.18 mg/kg,the contents of Mn and Cu were blow the detection limit. The counts of lactic acid bacteria were 2.49×108~2.98×109cfu/mL, the counts of yeast were 2.60×106~1.63×108cfu/mL.33 strains isolated from these samples belonged to Lactic acid bacteria . Among of 33 strains 7 were Lb. delbrueckii subsp. delbrueckii, 4 were Lb. dellbrueckii subsp. bulgaricus, 3 were Lb. acidophilus, 1 was Lb. coryniformis subsp. coryniformis, 10 were S. thermophilus, 5 were Ped. acidilactici and 1 for each of the Enterococcus faecalis,Enterococcus avium and Leu. mesenteroides subsp. mesenteroides respectively. The major lactic acid bacteria were S. thermophilus and Lb. delbrueckii subsp. delbrueckii.
【Key words】 Natural Fermented Goat milk; Chemical Composition; Microbial Composition; Lactic acid bacteria; Identification;
- 【网络出版投稿人】 内蒙古农业大学 【网络出版年期】2006年 10期
- 【分类号】TS252.1
- 【被引频次】17
- 【下载频次】395