【作者】 阿木尔吉日嘎拉；

【导师】 张七斤； 申之义； 关平原；

【作者基本信息】 内蒙古农业大学 ， 预防兽医学， 2006， 硕士

【摘要】 本研究用L.casei zhang和L.acidophilus MG2-1两株乳酸菌制成活菌数为2.0～3.0×1010cfu/ml的乳的悬浮液(菌液)和活菌数为2.0～3.0×108cfu/ml的发酵乳,并按不同剂量灌服小白鼠。在灌服的第5、10、15、20、25、30天6个时间点用ELISA试剂盒测定小白鼠血清IgG含量和肠粘膜粘液SIgA含量,选择第10、20、30天3个时间点用流式细胞仪测定小白鼠外周血T细胞CD3+、CD4+、CD8+亚群及其CD4+/CD8+比值的变化。结果表明:(1)灌服L.casei zhang和L.acidophilus MG2-1两株乳酸菌菌液及发酵乳的小白鼠血清中的IgG含量和肠粘膜粘液中SIgA含量较未饲喂乳酸菌的对照组明显提高(p<0.05,p<0.01),并且菌液组与发酵乳组之间、各剂量组之间也有显著差异(p<0.05,p<0.01)。随着时间的延长IgG含量和SIgA含量具有上升的趋势。(2)灌服L.casei zhang和L.acidophilus MG2-1两株乳酸菌菌液和发酵乳给小白鼠后均提高了小白鼠外周血中CD3+、CD4+、CD8+T细胞的比例,优化了T细胞CD4+/CD8+亚群的比值,数据统计处理结果显示,与未饲喂乳酸菌的对照组相比差异显著(p<0.05)或极显著(p<0.01)。说明这两株乳酸菌能够增强小白鼠的体液免疫功能和细胞免疫功能。用L.casei zhang和L.acidophilus MG2-1两株乳酸菌菌液和发酵乳以不同的剂量灌服给1日龄雏鸡,在10日龄和28日龄进行两次新城疫疫苗(IV系)滴鼻点眼免疫,在二免后的第7天和14天检测血凝抑制(HI)效价。结果表明,与未饲喂乳酸菌的对照组相比,试验各组抗体水平均提高到显著或极显著水平(p<0.05,p<0.01),可提高抗体水平0.61～1.77(log2)。说明这两株乳酸菌与新城疫疫苗有很好的协同作用,能够促进雏鸡免疫功能。更多还原

【Abstract】 In this research, the mice were fed with the milk suspension(2.0~3.0×1010 cfu/ml) and fermented milk(2.0~3.0×108 cfu/ml) which were made of Lactic Acid Bacteria L.casei zhang and L.acidophilus MG2-1 by different dosages. Use ELISA method to determine IgG content in the blood and SIgA content in the gut at 5th, 10th, 15th, 20th, 25th, 30th intervals, use FCM to determine the T cell CD3+, CD4+,CD8+ in the blood and calculate the change of CD4+/CD8+ ratio. The result showed that: (1) the IgG content in the blood and SIgA content in the gut of the mice which were fed with Lactic Acid Bacteria L. casei.zhang and L.acidophilus MG2-1 were significantly increased compared with the mice which were not fed(p<0.05, p<0.01), and there was also some significant differences between milk suspension groups, fermented milk groups and the different dosages (p<0.05, p<0.01). SIgA and IgG content have the tendency that was increased with time. (2) the proportion of T cell CD3+, CD4+,CD8+ in the blood was increased after fed with the milk suspension and fermented milk which were made of Lactic Acid Bacteria L.casei zhang and L.acidophilus MG2-1, optimize the CD4+/CD8+ ratio (p<0.05, p<0.01).Then we did another research, the chick were fed with milk suspension and fermented milk which were made of Lactic Acid Bacteria L.casei zhang and L.acidophilus MG2-1 by different dosages. The chick were vaccinated with ND two times at 10 days age and 28 days age, then determined the valence of HI antibody at 7th, 14th day after second vaccination. The result showed that compare with CK (did not fed with Lactic Acid Bacteria L.casei zhang and L.acidophilus MG2-1), the antibody level of each experimental group was all reached to significant and extremely significant (p<0.05, p<0.01), and the antibody level was enhanced by 0.61~1.77(log2).更多还原