【作者】 宁立娜；

【导师】 贾青；

【作者基本信息】 河北农业大学 ， 动物遗传育种与繁殖， 2006， 硕士

【摘要】 为了提高山羊繁殖性能,以增加山羊规模化生产经济效益,为高繁殖力山羊标记辅助育种提供基础材料,以河北中部山羊地方类群(冀中山羊)不同繁殖率群体为试验对象,对FSHR基因的部分区域的DNA序列特征进行了分析。 对冀中山羊的FSHR基因的5’侧翼调控区进行了扩增和测序,片段长度为967bp。对冀中山羊、波尔山羊和关中奶山羊的相应序列进行同源性分析,冀中山羊与关中奶山羊的序列同源性比冀中山羊和波尔山羊的同源性强,与三种山羊的亲缘关系相符。并且对扩增片段进行调控元件的检索,发现了10种调控元件,其中三个山羊品种的Y-box和PUC颠倒序列发生了变异,推测可能与冀中山羊的高产性能有关。用taq1对扩增片段进行了PCR-RFLP分析,没有发现多态性。 参照GeneBank发表的牛FSHR基因的cDNA序列,自行设计引物首次将山羊品种的FSHR基因的EXON10部分序列克隆、测序成功,共733bp。比较了冀中山羊、绵羊和牛的相应序列,发现冀中山羊和绵羊序列间的同源性较冀中山羊与牛序列间的同源性强。并将EXON10序列翻译成氨基酸序列,比较发现了4处变异,其中牛的第660位的丝氨酸,而山羊和绵羊为苯丙氨酸,这可能与它们的高繁殖力有关。用DNAStar软件对冀中山羊、绵羊、牛、猪、人和猩猩的FSHR基因的EXON10序列做性同源性比较分析和进化树分析,所得结果与物种间的进化关系相符。 对冀中山羊抽样群体FSHR基因的EXON10部分序列进行PCR-SSCP基因型判定,发现了两种基因型:AB型和AA型。高产群和普通群体的从基因型频率都比相应的低产群要低,可推测等位基因B对冀中山羊高繁殖性能有正向作用,而A基因对冀中山羊的繁殖性能有负向效应。对不同基因型个体测序,考察第1506位有单碱基C→T,AB型个体为C,AA型个体为T,推测第1506位的碱基C对家畜产仔数性状为正向突变。更多还原

【Abstract】 To improve the number of the livestock’s babies can improve economic benefit. In this study the characteristic analysis of the partial region of FSHR gene was made in different prolificacy goats in the middle region of Hebei province, which can offer basic matetial of Marker Assistant Breeding in prolific goats.FSHR gene’s of Jizhong goats was amplified and sequenced , the fragment of Jizhong-goat was 967bp. Alignment analysis in Jizhong, Boer and Guanzhong goats showed that the nucleotide sequence identity of Jizhong-goat and Guanzhong-goat was higher than that of Jizhong-goat and Boer goat, which was consistent with blood relationship of the three species goats. Scanning the 5’flanking regulating region of FSHR gene discovered 10 regulating elements, and Y-box sequence and PUC reverse sequence had variation in the three species goats, and it was conferred that this mutation was related with the prolificacy of Jizhong-goat. Using PCR-RFLP method to analyze the differentiation of the amplified sequence by digesting it with taql, the mutation was not found.According to the reported sequence of cattle’s cDNA of FSHR gene from GeneBank, the primer was designed and the FSHR gene’s EXONIO sequence of Jizhong-goat was cloned and sequenced, the fragment was 733bp. It was showed that the identity of Jizhong-goat and sheep was higher than that of Jizhong-goat and cattle by comparing the different sequences of Jizhong-goat, sheep and cow. The sequence of the FSHR gene’s EXON10 was translated to amino acid sequence;it was found 4 mutant loci and the His amino acid of cow in 660 loci maybe take important role in monovular cow. Identity analysis and Phylogenetic Tree analysis was made in Jizhong-goat, sheep, cow, swine, human and ape, and what indicated was similar as their evolution relationship.The part of FHSR gene’s EXONIO region was analyzed using PCR-RFLP method, and found two genotypes: AB and AA. The frequency of genotype AA in prolific group and normal group were lower than low prolificacy group. It can be inferred that gene B made positive effect in prolificacy in Jizhong-goat, and gene A made negative effect in prolificacy. The sequences of different genotypes were sequenced and found one mutation: C→T. Genotype AB’s sequence was C and genotype AA’s sequence was T, and it can be inferred that base C in 1506 loci was a positive mutation in fecundity traits.更多还原